Performance of the Xpert HBV Viral Load assay versus the Aptima Quant assay for quantifying hepatitis B virus DNA

Autor: Catherine Haslé, Florence Abravanel, Jacques Izopet, Agnès Harter, Stéphanie Raymond, Marion Migueres, Sébastien Lhomme, Laurent Alric, Pauline Trémeaux, Patrick Bruel, Sophie Metivier
Přispěvatelé: Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), CCSD, Accord Elsevier, Laboratoire Virologie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)
Rok vydání: 2020
Předmět:
0301 basic medicine
[SDV]Life Sciences [q-bio]
MESH: Molecular Diagnostic Techniques
medicine.disease_cause
Workflow
MESH: Genotype
0302 clinical medicine
Hbv genotype
Medicine
030212 general & internal medicine
MESH: Viral Load / standards
GeneXpert MTB/RIF
Clinical performance
virus diseases
General Medicine
Viral Load
Hepatitis B
3. Good health
[SDV] Life Sciences [q-bio]
MESH: Reproducibility of Results
Clinical Practice
Infectious Diseases
Molecular Diagnostic Techniques
Molecular diagnosis
Viral load
MESH: Hepatitis B / diagnosis
Microbiology (medical)
Hepatitis B virus
Genotype
030106 microbiology
Hepatitis B virus DNA
Sensitivity and Specificity
MESH: Workflow
03 medical and health sciences
MESH: Hepatitis B virus / genetics
Humans
MESH: Hepatitis B / virology
Antiviral treatment
MESH: Humans
business.industry
Reproducibility of Results
Virology
MESH: Sensitivity and Specificity
MESH: Viral Load / methods
digestive system diseases
MESH: DNA
Viral
DNA
Viral
business
GeneXpert instrument
Zdroj: Diagnostic Microbiology and Infectious Disease
Diagnostic Microbiology and Infectious Disease, Elsevier, 2020, 96, pp.114946-. ⟨10.1016/j.diagmicrobio.2019.114946⟩
Diagnostic Microbiology and Infectious Disease, 2020, 96 (2), pp.1-7. ⟨10.1016/j.diagmicrobio.2019.114946⟩
ISSN: 0732-8893
1879-0070
DOI: 10.1016/j.diagmicrobio.2019.114946
Popis: International audience; Quantification of HBV DNA is used for initiating and monitoring antiviral treatment. We have evaluated the Xpert HBV Viral Load (VL) assay on the GeneXpert instrument. We estimated its limit of detection to be 7.5 IU/mL. Reproducibility was 1.1-12.7% as assessed by the coefficients of variation for 3 different samples. The assay was linear from 2 to 8 log10 IU/mL for HBV genotypes A to F. Its clinical performance was evaluated by testing prospectively 100 HBV DNA-positive samples with the Xpert HBV VL and Aptima Quant HBV assays. The results from the 2 assays were correlated, with a modest bias (-0.10 log10 IU/mL) between them by Bland-Altman analysis. Patient monitoring with 80 samples performed with both assays gave similar patient profiles with trends in the same direction. The Xpert HBV Viral load assay is reliable enough for quantifying HBV DNA in clinical practice.
Databáze: OpenAIRE
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