Characterization of N-Linked Oligosaccharides Attached to Human Renin Expressed in COS Cells
| Autor: | Yasuyuki Ishizuka, Hitoshi Hori, Hideaki Umeyama, Teruhiko Yoshino, Yasuhiko Shiratori, Setsuko Nakagawa, Takeshi Yamauchi, Kazuo Murakami |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
chemistry.chemical_classification
COS cells Mutant Oligosaccharides Heterologous Mutagenesis (molecular biology technique) Biology Oligosaccharide Transfection Molecular biology Cell Line Enzyme Biochemistry chemistry Mutation Renin Internal Medicine Humans Secretion DNA Circular Site-directed mutagenesis |
| Zdroj: | Clinical and Experimental Hypertension. Part A: Theory and Practice. 10:1147-1155 |
| ISSN: | 0730-0077 |
| DOI: | 10.1080/07300077.1988.11878807 |
| Popis: | To study the role of N-linked oligosaccharides attached to human renin, we generated three kinds of glycosylation-deficient renins in which one or both of two putative N-glycosylation sites was eliminated by amino acid replacement using site-directed mutagenesis. Examination of the three mutant renins (Asn-5 to Ala, Asn-75 to Ala, and both Asn-5 and -75 to Ala) expressed in COS cells demonstrated that both putative sites were certainly glycosylated with heterologous N-linked oligosaccharides. Moreover, the oligosaccharide chain attached at Asn-5 was different from that attached at Asn-75 in its molecular size. In addition, the secreted amount of the three mutant renins were different from one another, although the mutant and wild-type renins had practically the same specific activity. Our results suggest that the N-linked oligosaccharides have no effect on the enzymatic activity, but play an important role in stable secretion of human renin. |
| Databáze: | OpenAIRE |
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