Differences in Humoral Insulin-Antibody Response Among Inbred Lou/M Rats and Epitope Presentation Differences in ELISA and Radioimmune Titration
| Autor: | Sandy Edwards, David P Stenger, Lourdes Mosqueda, Brenda R McDougall, Edward R. Arquilla |
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| Rok vydání: | 1989 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Radioimmunoassay Enzyme-Linked Immunosorbent Assay Biology Epitope Epitopes Immune system Internal medicine Internal Medicine medicine Animals Insulin Antiserum Rats Inbred Strains Molecular biology Rats Titer Endocrinology Antibody Formation biology.protein Titration Antibody |
| Zdroj: | Diabetes. 38:868-873 |
| ISSN: | 1939-327X 0012-1797 |
| Popis: | Insulin antibodies were not detected in an insulin-capture enzyme-linked immunosorbent assay (ELISA), but they were easily detected in an insulin-copolymer-capture ELISA. Thus, there is a high degree of steric hindrance because of the proximity of the epitopes on the insulin monomer. This is circumvented by substituting an insulin copolymer for insulin in the capture ELISA. A regression analysis comparing the titers of 28 Lou/M rat insulin antiserums measured by liquid-phase radioimmune titration (RIT) with titers obtained in the direct insulin ELISA was not significant (P >.05). Thus, epitopes on insulin available and/or masked for antibody binding in the RIT differ from those available and/or masked in the direct insulin ELISA. As more of the epitopes become available when an insulin copolymer is substituted for monomeric insulin in the ELISAs, a significant positive correlation (P |
| Databáze: | OpenAIRE |
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