Protection of Cotton Rats against Human Respiratory Syncytial Virus by Vaccination with a Novel Chimeric FG Glycoprotein
| Autor: | M. A. Stier, R. R. Walters, M. W. Wathen, Roger J. Brideau |
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| Rok vydání: | 1989 |
| Předmět: |
Paramyxoviridae
Recombinant Fusion Proteins viruses Dose-Response Relationship Immunologic Respirovirus Infections Virus Neutralization Microbiology Adjuvants Immunologic Viral Envelope Proteins Virology Animals Cotton rat Neutralizing antibody Immunization Schedule Glycoproteins chemistry.chemical_classification Vaccines Vaccines Synthetic biology Arvicolinae Viral Vaccines biology.organism_classification Respiratory Syncytial Viruses Vaccination chemistry biology.protein Antibody Glycoprotein |
| Zdroj: | Journal of General Virology. 70:2637-2644 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-70-10-2637 |
| Popis: | Summary The cotton rat model of experimental human respiratory syncytial virus (RSV) infection was used to study the efficacy of FG, a novel chimeric glycoprotein which was expressed in insect cells using a baculovirus vector. FG contained the extracellular regions of the F (fusion) and G (attachment) glycoproteins of RSV. Vaccination with FG resulted in induction of neutralizing antibody and was correlated with protection of lung tissue from RSV challenge against both serogroup A and B virus strains. Both crude FG taken from supernatants of insect cells and affinity-purified FG were immunogenic and active against RSV. FG vaccination was effective by three routes of administration, following a single dose, and when administered with different adjuvants. |
| Databáze: | OpenAIRE |
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