Histone variant Htz1 promotes histone H3 acetylation to enhance nucleotide excision repair in Htz1 nucleosomes
| Autor: | Yanbo Deng, Yachuan Yu, Catherine B. Millar, R. Waters, Simon H. Reed |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Saccharomyces cerevisiae Proteins
DNA Repair Ultraviolet Rays Lipoproteins Saccharomyces cerevisiae Genome Integrity Repair and Replication Chromatin remodeling Pheromones Histones 03 medical and health sciences 0302 clinical medicine Histone H1 Histone H2A Histone methylation Genetics Histone code Histone H3 acetylation Promoter Regions Genetic QH426 030304 developmental biology Histone Acetyltransferases Adenosine Triphosphatases 0303 health sciences Microbial Viability biology Acetylation Molecular biology Chromatin Nucleosomes enzymes and coenzymes (carbohydrates) Histone DNA Repair Enzymes Pyrimidine Dimers biology.protein 030217 neurology & neurosurgery Gene Deletion DNA Damage |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Nucleotide excision repair (NER) is critical for maintaining genome integrity. How chromatin dynamics are regulated to facilitate this process in chromatin is still under exploration. We show here that a histone H2A variant, Htz1 (H2A.Z), in nucleosomes has a positive function in promoting efficient NER in yeast. Htz1 inherently enhances the occupancy of the histone acetyltransferase Gcn5 on chromatin to promote histone H3 acetylation after UV irradiation. Consequently, this results in an increased binding of a NER protein, Rad14, to damaged DNA. Cells without Htz1 show increased UV sensitivity and defective removal of UV-induced DNA damage in the Htz1-bearing nucleosomes at the repressed MFA2 promoter, but not in the HMRa locus where Htz1 is normally absent. Thus, the effect of Htz1 on NER is specifically relevant to its presence in chromatin within a damaged region. The chromatin accessibility to micrococcal nuclease in the MFA2 promoter is unaffected by HTZ1 deletion. Acetylation on previously identified lysines of Htz1 plays little role in NER or cell survival after UV. In summary, we have identified a novel aspect of chromatin that regulates efficient NER, and we provide a model for how Htz1 influences NER in Htz1 nucleosomes. |
| Databáze: | OpenAIRE |
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