Intracellular SERS hybrid probes using BSA–reporter conjugates
| Autor: | Sabine Flemig, Daniela Drescher, Janina Kneipp, Andrea Hornemann |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Biocompatibility
Cells Metal Nanoparticles Nanotechnology Biosensing Techniques Endosomes Spectrum Analysis Raman Biochemistry Analytical Chemistry Mice Nanosensor Animals Molecule Bovine serum albumin chemistry.chemical_classification biology Biomolecule Serum Albumin Bovine chemistry Colloidal gold Covalent bond Molecular Probes NIH 3T3 Cells biology.protein Cattle Conjugate |
| Zdroj: | Analytical and Bioanalytical Chemistry. 405:6209-6222 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-013-7054-3 |
| Popis: | Surface-enhanced Raman scattering (SERS) hybrid probes are characterized by the typical spectrum of a reporter molecule. In addition, they deliver information from their biological environment. Here, we report SERS hybrid probes generated by conjugating different reporter molecules to bovine serum albumin (BSA) and using gold nanoparticles as plasmonic core. Advantages of the BSA-conjugate hybrid nanoprobes over other SERS nanoprobes are a high biocompatibility, stabilization of the gold nanoparticles in the biological environment, stable reporter signals, and easy preparation. The coupling efficiencies of the BSA-reporter conjugates were determined by MALDI-TOF-MS. The conjugates' characteristic SERS spectra differ from the spectra of unbound reporter molecules. This is a consequence of the covalent coupling, which leads to altered SERS enhancement and changes in the chemical structures of the reporter and of BSA. The application of the BSA-reporter conjugate hybrid probes in 3T3 cells, including duplex imaging, is demonstrated. Hierarchical cluster analysis and principal components analysis were applied for multivariate imaging using the SERS signatures of the incorporated SERS hybrid nanoprobes along with the spectral information from biomolecules in endosomal structures of cells. The results suggest more successful applications of the SERS hybrid probes in cellular imaging and other unordered high-density bioanalytical sensing. |
| Databáze: | OpenAIRE |
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