Delayed type hypersensitivity induced by intradermal inoculation of a Neospora caninum tachyzoite antigen in previously exposed cattle
| Autor: | Maria Rosa Leunda, Y.P. Hecker, Anselmo Carlos Odeón, P.M. Corva, Susana Beatriz Pereyra, Franco Fiorani, Germán J. Cantón, Dadin Prando Moore, Virginia Aráoz, Joaquín Ignacio Armendano |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Injections
Intradermal 040301 veterinary sciences Immunology Dose-Response Relationship Immunologic Cattle Diseases Antigens Protozoan Biology Serology Microbiology 0403 veterinary science 03 medical and health sciences Antigen Immunity parasitic diseases Animals Hypersensitivity Delayed 030304 developmental biology 0303 health sciences General Veterinary Coccidiosis Inoculation Ciencias Veterinarias fungi Neospora 04 agricultural and veterinary sciences biology.organism_classification Neospora caninum Delayed type hypersensitivity Specific antibody CIENCIAS AGRÍCOLAS Immunoglobulin G biology.protein Protozoa Cattle Antibody |
| Popis: | The aim of this study was to evaluate delayed type hypersensitivity (DTH) induced by the intradermal inoculationof a Neospora caninum tachyzoite soluble lysate in cattle previously exposed with the protozoa. Fourexperimental groups were selected according to the prior exposure to N. caninum antigen. All cows were intradermallyinjected with a N. caninum tachyzoite soluble lysate and skinfold thickness growth at the inoculationsites was measured at 0, 24, 48, 72 and 96 h post inoculation (hpi). Additionally, specific antibodies and IFN-γproduction were assessed. Cows experimentally infected with live N. caninum tachyzoites and cows naturallyexposed to N. caninum developed skin reactions compatible with DTH between 24 and 96 hpi (p < 0.05).Moreover, cows inoculated with an experimental N. caninum vaccine and cows without evidence of exposure toN. caninum did not show a significant increase in skin thickness (p > 0.05). Furthermore, serological status ofthe animals was not modified due to the intradermal inoculation. The highest IFN-γ production was observed at15 days after intradermal inoculation (p < 0.05). Therefore, these results suggest that cattle previously exposedto N. caninum develop a reaction compatible with DTH which could be useful as in vivo cell mediated immunityparameter for assessed bovine neosporosis. Fil: Fiorani, Franco. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina Fil: Armendano, Joaquín Ignacio. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Araoz, Virginia. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Cantón, Germán J.. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Leunda, María R.. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Pereyra, Susana B.. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Corva, Pablo M.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Odeón, Anselmo C.. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Moore, Dadín P.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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