Changes in the amounts of chondroitin sulfate proteoglycans in rat brain after neonatal hypoxia-ischemia
| Autor: | Masako Nishizuka, Yoshihito Tokita, Hiroomi Keino, Kanako Hirano, Hiroko Kakizawa, Fumiko Matsui, Atsuhiko Oohira, Sachiko Aono, Michiru Ida, Takuya Shuo |
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| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty Blotting Western Central nervous system Ischemia Biology Rats Sprague-Dawley Cellular and Molecular Neuroscience Western blot Neurocan Internal medicine medicine Animals Brain Chemistry Cerebral Cortex Habenula medicine.diagnostic_test Receptor-Like Protein Tyrosine Phosphatases Class 5 Perineuronal net Chondroitin Sulfates Membrane Proteins Hypoxia (medical) medicine.disease Immunohistochemistry Rats Carotid Arteries medicine.anatomical_structure Endocrinology Animals Newborn Chondroitin Sulfate Proteoglycans Hypoxia-Ischemia Brain Immunology Proteoglycans medicine.symptom Immunostaining |
| Zdroj: | Journal of Neuroscience Research. 81:837-845 |
| ISSN: | 1097-4547 0360-4012 |
| DOI: | 10.1002/jnr.20603 |
| Popis: | Chondroitin sulfate proteoglycans have been shown to participate in the pathogenesis of neuronal damages in the injured adult central nervous system (CNS). Upregulated expression of chondroitin sulfate proteoglycans has been reported around the injured sites and depletion of these chondroitin sulfate proteoglycans brings about increased axonal regeneration in the injured adult CNS. To examine if chondroitin sulfate proteoglycans are also involved in the pathologic process of hypoxia-ischemia in the neonatal brain, expressions of three chondroitin sulfate proteoglycans, neurocan, phosphacan, and neuroglycan C, were examined in rat brains after neonatal hypoxia-ischemia. Hypoxic-ischemic rats were produced by ligating the right carotid artery of 7-day-old rats, followed by 8% oxygen exposure. Western blot analysis revealed that in contrast to injured adult CNS, the amount of neurocan was reduced 24 hr after hypoxia in the neonatal hypoxic-ischemic cerebral hemisphere. The amounts of phosphacan and neuroglycan C were also reduced significantly 24 hr after hypoxia at the right injured cortex compared to those at the left cortex. Surprisingly, the immunohistologic staining for phosphacan was conversely intensified both at 24 hr and 8 days after hypoxia at the infarcted area. In addition, the habenula and fascicules retroflexus in the right cerebral hemisphere degenerated and became intensely immunostained with the anti-phosphacan antibody shortly after hypoxia. Hypoxic-ischemic insult may unmask phosphacan epitopes at the injured sites, resulting in intensified immunostaining. Because intensified immunostaining for neurocan and neuroglycan C was not observed, unmasking seems to be specific to phosphacan among these three chondroitin sulfate proteoglycans. |
| Databáze: | OpenAIRE |
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