Plasmodium berghei-infection induces volume-regulated anion channel-like activity in human hepatoma cells
| Autor: | Henry M. Staines, Elvira T. Derbyshire, Catarina A. Marques, Maria M. Mota, Miguel Prudêncio, Sanjeev Krishna |
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| Rok vydání: | 2009 |
| Předmět: |
Anions
Cell Membrane Permeability Plasmodium berghei Immunology Microbiology Ion Channels Clomiphene Ion 03 medical and health sciences 0302 clinical medicine Virology Animals Humans Enzyme Inhibitors Ion channel Cell Size 030304 developmental biology 0303 health sciences biology Conductance Original Articles biology.organism_classification Molecular biology 3. Good health Mefloquine Tamoxifen Hepatoma cell line Membrane Biochemistry Permeability (electromagnetism) Nitrobenzoates 030220 oncology & carcinogenesis Hepatocytes Tonicity Chlorine |
| Zdroj: | Cellular Microbiology |
| ISSN: | 1462-5822 1462-5814 |
| DOI: | 10.1111/j.1462-5822.2009.01342.x |
| Popis: | Parasite infection can lead to alterations in the permeability of host plasma membranes. Presented here is the first demonstration that this phenomenon occurs in Plasmodium-infected liver cells. Using the whole-cell patch-clamp technique, volume-regulated anion channel (VRAC) activity was characterized in Huh-7 cells (a human hepatoma cell line) before and after infection with Plasmodium berghei. Consistent with the presence of VRACs, hypotonic bath solution induced large ion currents in Huh-7 cells that rectified outwardly, reversed close to the equilibrium potential for Cl(-) and were inhibited by tamoxifen, clomiphene, mefloquine and 5-nitro-2, 3-(phenylpropylamino)-benzoic acid (NPPB), with IC(50) values of 4 +/- 1, 4 +/- 2, 2 +/- 1 and 52 +/- 12 microM respectively. In isotonic conditions, initial current recordings measured in uninfected and immature (24 h post invasion) parasite-infected Huh-7 cells were similar (with conductances of 14 +/- 3 versus 19 +/- 5 pS/pF). However, in mature (48-72 h post invasion) parasite-infected Huh-7 cells there was a sevenfold increase in currents (with a conductance of 98 +/- 16 pS/pF). The elevated currents observed in the latter are consistent with VRAC-like activity and the possible reasons for their activation are discussed. |
| Databáze: | OpenAIRE |
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