Coexpressed Auxiliary Subunits Exhibit Distinct Modulatory Profiles on AMPA Receptor Function
| Autor: | Alexandru Rusu, Ling Zhang, Jakob von Engelhardt, Paul Farrow, Anton Schulmann, Konstantin Khodosevich, Eric Jacobi, Rolf Sprengel, Hannah Monyer |
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| Rok vydání: | 2014 |
| Předmět: |
Cell type
physiology [Excitatory Postsynaptic Potentials] Patch-Clamp Techniques Neuroscience(all) Cell Nerve Tissue Proteins AMPA receptor Gating genetics [Neuronal Plasticity] Biology Synaptic Transmission Gene Knockout Techniques Mice methods [Patch-Clamp Techniques] medicine physiology [Neuronal Plasticity] Animals Humans ddc:610 Receptors AMPA Neurons metabolism [Nerve Tissue Proteins] CKAMP44 protein mouse Neuronal Plasticity General Neuroscience Dentate gyrus musculoskeletal neural and ocular physiology metabolism [Receptors AMPA] metabolism [Calcium Channels] Excitatory Postsynaptic Potentials Membrane Proteins Long-term potentiation metabolism [Dentate Gyrus] Receptor Desensitization physiology [Neurons] Cell biology Electrophysiology medicine.anatomical_structure nervous system metabolism [Neurons] physiology [Synaptic Transmission] Dentate Gyrus Synapses genetics [Receptors AMPA] Calcium Channels physiology [Synapses] metabolism [Membrane Proteins] TARP gamma-8 protein mouse |
| Zdroj: | Neuron 83(3), 601-615 (2014). doi:10.1016/j.neuron.2014.07.004 Neuron |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/j.neuron.2014.07.004 |
| Popis: | Summary Gating properties and surface trafficking of AMPA receptors (AMPARs) are modulated by auxiliary subunits. Here we studied the function of coexpressed auxiliary subunits belonging to two different classes. We focused on TARP γ-8 and CKAMP44 in dentate gyrus (DG) granule cells, since both subunits are highly expressed in this cell type. TARP γ-8 and CKAMP44 decrease the rate of deactivation but have an opposing influence on receptor desensitization, which accounts for their differential modulation of synaptic short-term plasticity. Furthermore, long-term plasticity (LTP) requires TARP γ-8 but not CKAMP44. The coexpression of both auxiliary subunits is necessary for the efficient targeting of AMPARs to the cell surface of DG granule cells. Finally, electrophysiological and biochemical evidence support the notion that CKAMP44 and TARP γ-8 can be contained in the same AMPAR complex. Video Abstract |
| Databáze: | OpenAIRE |
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