Sub-chronic iron overload triggers oxidative stress development in rat brain: implications for cell protection
| Autor: | Juan Carlos Perazzo, Virginia Fernández, Luis A. Videla, Natacha E Piloni, Susana Puntarulo |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD Iron Overload Antioxidant Free Radicals Iron medicine.medical_treatment Ciencias de la Salud Hippocampus Striatum medicine.disease_cause Antioxidants General Biochemistry Genetics and Molecular Biology Biomaterials Superoxide dismutase 03 medical and health sciences Downregulation and upregulation Internal medicine medicine Animals Nf-Κb biology Superoxide Dismutase Chemistry Metals and Alloys Brain Catalase Rats Cortex (botany) Otras Ciencias de la Salud Oxidative Stress 030104 developmental biology Endocrinology biology.protein Iron-Dextran Complex General Agricultural and Biological Sciences Oxidative stress |
| Zdroj: | BioMetals. 29:119-130 |
| ISSN: | 1572-8773 0966-0844 |
| Popis: | This work was aimed to test the hypothesis that sub-chronic administration of iron-dextran (Fe-dextran) (six doses of 50 mg Fe-dextran/kg) to rats triggers a transient oxidative stress in brain and mechanisms of cellular antioxidant defence. After 2 h of administration of the 6th dose, a significant increase of total Fe, the labile Fe pool (LIP), the lipid radical (LR•)/α-tocopherol (α-T) content ratio were observed, as compared to values in control brain homogenates. The ascorbyl radical (A•)/ascorbate (AH-) content ratio and the oxidation rate of 2′,7′-dichlorodihidrofluorescein (DCFH-DA) were significantly higher in Fe-dextran treated rats, as compared to values in brain from control rats after 4 h treatment. An increase in both catalase (CAT) and superoxide dismutase (SOD) activity was observed at 8 and 1-2 h, respectively. No significant changes were detected in the nuclear factor-κB (NF-κB) levels in nuclear extracts from rat brains after 1-8 h of Fe-dextran administration. After 2 h of Fe administration Fe concentration in cortex, striatum and hippocampus was significantly increased as compared to the same areas from control animals. Both, CAT and SOD activities were significantly increased in cortex after Fe administration over control values, without changes in striatum and hippocampus. Taken as a whole, sub-chronic Fe administration enhances the steady state concentration of Fe in the brain LIP that favors the settlement of an initial oxidative stress condition, both at hydrophilic and lipophilic compartments, resulting in cellular protection evidenced by antioxidant enzyme upregulation. Fil: Piloni, Natacha Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina Fil: Perazzo, Juan C.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Fernandez, Virginia. Universidad de Chile; Chile Fil: Videla, Luis A.. Universidad de Chile; Chile Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina |
| Databáze: | OpenAIRE |
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