Cultivation of Mycobacterium paratuberculosis from bovine fecal specimens and a suggested standardized procedure
| Autor: | Diana L. Whipple, Jarnagin Jl, Donald R. Callihan |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
0301 basic medicine
Fastidious organism Tuberculosis 040301 veterinary sciences 030106 microbiology Paratuberculosis Cattle Diseases Microbiology Enteritis 0403 veterinary science Mycobacterium tuberculosis 03 medical and health sciences Feces medicine Animals General Veterinary biology 04 agricultural and veterinary sciences biology.organism_classification Isolation (microbiology) medicine.disease Mycobacterium avium subsp. paratuberculosis Cattle Mycobacterium |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 3(4) |
| ISSN: | 1040-6387 |
| Popis: | Paratuberculosis is a chronic granulomatous enter- paratuberculosis from fecal specimens and to suggest itis of ruminants caused by a small, fastidious, acid- a standardized cultivation procedure. fast bacillus, Mycobacterium paratuberculosis. The disease now called paratuberculosis was described in 1826 as an enteritis found in some cattle with chronic diarrhea. 3 Johne and Frothingham further described the Review of the literature Isolation of the organism now known as M. paratuberculosis was first reported in 1910, 39 and the comdisease in 1895 and demonstrated the presence of acid- plete description of the procedure was reported in fast bacilli in affected intestine. 3 The first successful 1912.40 An inspissated egg-base medium containing isolation of this organism was in 1910 by Twort, who Mycobacterium tuberculosis was used. The authors innamed in Mycobacterium enteriditis chronicae pseu- cluded M. tuberculosis because they recognized that dotuberculosis bovis johne. 39,40 The name of the organ- some type of “necessary foodstuff needed to support ism has since been changed to Mycobacterium para- growth of M. paratuberculosis was missing from the tuberculosis, 1 and the clinical disease is called Johne’s medium. They theorized that this “foodstuff’ could disease. 3 be supplied by another acid-fast organism, such as M. Many tests have been developed to aid in diagnosis tuberculosis. After successfully isolating M. paratuberof paratuberculosis; however, isolation of M. paratu- culosis on egg medium containing M. tuberculosis, they berculosis from tissue or fecal specimens is regarded substituted the timothy grass bacillus (M. phlei) for M. as the definitive diagnostic test in cattle. 3,5 Cultivation tuberculosis and observed growth that was superior. of M. paratuberculosis from fecal specimens is the most The “necessary foodstuff’ was extracted from M. phlei frequently used diagnostic test and is the standard for using hot ethanol and was used in medium instead of a suspension of M. phlei to support growth of M. para |
| Databáze: | OpenAIRE |
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