IM-412 inhibits the invasion of human breast carcinoma cells by blocking FGFR-mediated signaling
| Autor: | Seung-Youn Jung, Jie-Young Song, Jiyeon Ahn, Jae Youn Yi, Sang-Gu Hwang, Mi-Hyoung Kim, Kyung Hee Song, Ky-Youb Nam, Seong-Mook Kang |
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| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Epithelial-Mesenchymal Transition Cellular differentiation Triple Negative Breast Neoplasms Biology Cancer stem cell Cell Movement Transforming Growth Factor beta Radioresistance Cell Line Tumor medicine Biomarkers Tumor Humans Receptor Fibroblast Growth Factor Type 3 Epithelial–mesenchymal transition Receptor Fibroblast Growth Factor Type 1 Phosphorylation Oncogene Imidazoles Cancer Cell Differentiation General Medicine Transforming growth factor beta medicine.disease Oncology Purines Cancer cell Immunology Cancer research biology.protein Female Signal Transduction |
| Zdroj: | Oncology reports. 34(5) |
| ISSN: | 1791-2431 |
| Popis: | Triple-negative breast cancer (TNBC) is an aggressive cancer with a poor prognosis due to its epithelial‑to-mesenchymal transition (EMT) phenotype. Cancer patients often experience several detrimental effects of cancer treatment, such as chemoresistance, radioresistance and the maintenance of cancer stem cells due to EMT. Thus, EMT signaling is considered to be a valuable therapeutic target for cancer treatment, and its inhibition is being attempted as a new treatment option for TNBC patients. Previously, we showed that 3-(2-chlorobenzyl)-1,7-dimethyl-1H-imidazo[2,1-f]purine‑2,4(3H,8H)-dione (IM-412) inhibits transforming growth factor-β (TGF-β)-induced differentiation of human lung fibroblasts through both Smad-dependent and -independent pathways. In the present study, we examined the inhibitory effect of IM-412 on EMT pathways and invasiveness in TNBC cells since the TGF-β signaling pathway is a typical signaling pathway that functions in EMT. IM-412 not only potently suppressed the migration and invasion of MDA-MB-231 cells, but also lowered the expression of mesenchymal markers and EMT-activating transcription factors in these cells. IM-412 inhibited the activation of several signaling proteins, including Smad2/Smad3, p38MAPK, Akt and JNK, and it also attenuated the phosphorylation of FGFR1 and FGFR3. Collectively, our findings suggest that the synthetic compound IM-412 suppressed the EMT process in MDA-MB-231 cells and thereby effectively inhibited the migration and invasion of these cancer cells. Thus, IM-412 could serve as a novel therapeutic agent for malignant cancers. |
| Databáze: | OpenAIRE |
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