Understanding the regulatory relationship of abscisic acid and bZIP transcription factors towards amylose biosynthesis in wheat
| Autor: | Himanshu Sharma, Afsana Parveen, Joy Roy, Prashant Kumar, Vikas Rishi, Mohammed Saba Rahim, Pankaj Kumar, Koushik Shah, Ankita Mishra |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Mutant Response Elements Transcriptome 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Amylose Gene Expression Regulation Plant Arabidopsis Gene expression Genetics Molecular Biology Gene Abscisic acid Transcription factor Genetic Association Studies Triticum Binding Sites biology Base Sequence food and beverages Starch General Medicine biology.organism_classification Biosynthetic Pathways 030104 developmental biology Basic-Leucine Zipper Transcription Factors Biochemistry chemistry 030220 oncology & carcinogenesis Mutation Abscisic Acid Signal Transduction |
| Zdroj: | Molecular biology reports. 48(3) |
| ISSN: | 1573-4978 |
| Popis: | Starch is biosynthesized during seed development and this process is regulated by many bZIP proteins in bread wheat. Abscisic acid (ABA), an important phyto-hormone involved in various physiological processes mediated by bZIPs in plants including seed development. The ‘Group A’ TabZIP transcription factors play important roles in the ABA signaling pathway in Arabidopsis, rice and other cereal crops but their role in regulation of amylose biosynthesis in wheat is limited. In this study 83 ‘Group A’ TabZIPs were characterized by gene expression analysis in wheat amylose mutants. A set of 17 TabZIPs was selected on the basis of differential expression (> 2 fold) in low and high amylose mutants from RNA-seq data and validated by qRT PCR. Based on qRT PCR and correlation analysis out of the 17 TabZIPs six differentially expressed candidate TabZIPs were identified, involving in high amylose biosynthesis. The TabZIP175.2, identified as upregulated in all high amylose lines and TabZIP90.2, TabZIP129.1, TabZIP132.2, TabZIP143 and TabZIP159.2 were found downregulated in all low amylose lines, after exogenous supply of ABA. Proximal promoter analysis of starch pathway genes revealed the presence of ABA-responsive elements (ABREs) that are putative binding sites for bZIPs. Collectively, these findings indicated the involvement of putative six candidate TabZIPs as transcriptional regulators of amylose related genes via an ABA-dependent pathway in wheat. This study could help the investigators to make an informed decision to edit wheat genome for high/low amylose content using gene-editing technologies. |
| Databáze: | OpenAIRE |
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