Endocannabinoid regulation of spinal nociceptive processing in a model of neuropathic pain

Autor:	Javier Fernández-Ruiz, Eva de Lago, Maulik D. Jhaveri, Devi Rani Sagar, Royston A. Gray, David A. Barrett, Victoria Chapman, Denise Richardson, David A. Kendall
Rok vydání:	2010
Předmět:	Male AM251 Cannabinoid receptor Polyunsaturated Alkamides medicine.medical_treatment TRPV1 TRPV Cation Channels Arachidonic Acids Pharmacology Rats Sprague-Dawley Receptor Cannabinoid CB2 Piperidines Receptor Cannabinoid CB1 Evoked Potentials Somatosensory Physical Stimulation Cannabinoid Receptor Modulators medicine Cannabinoid receptor type 2 Animals Anesthesia Furans Neurons Camphanes business.industry General Neuroscience Spinal cord Rats Disease Models Animal Allodynia medicine.anatomical_structure Spinal Cord Neuropathic pain Neuralgia Pyrazoles Cannabinoid medicine.symptom business Microelectrodes Neuroscience Central Nervous System Agents Endocannabinoids medicine.drug
Zdroj:	European Journal of Neuroscience. 31:1414-1422
ISSN:	1460-9568 0953-816X
DOI:	10.1111/j.1460-9568.2010.07162.x
Popis:	Models of neuropathic pain are associated with elevated spinal levels of endocannabinoids (ECs) and altered expression of cannabinoid receptors on primary sensory afferents and post-synaptic cells in the spinal cord. We investigated the impact of these changes on the spinal processing of sensory inputs in a model of neuropathic pain. Extracellular single-unit recordings of spinal neurones were made in anaesthetized neuropathic and sham-operated rats. The effects of spinal administration of the cannabinoid CB(1) receptor antagonist N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251) and the cannabinoid receptor type 2 (CB(2)) receptor antagonist N-[(1S)-endo-1,3,3-trimethylbicycloheptan-2-yl]-5-(4-chloro-3-methylphenyl)-1-(4-methylbenzyl)-pyrazole-3-carboxamide (SR144528) on mechanically-evoked responses of spinal neurones were determined. The effects of spinal administration of (5Z,8Z11Z,14Z)-N-(3-furanylmethyl)-5,8,11,14-eicosatetraenamide (UCM707), which binds to CB(2) receptors and alters transport of ECs, on evoked responses of spinal neurones and spinal levels of ECs were also determined. The cannabinoid CB(1) receptor antagonist AM251, but not the CB(2) receptor antagonist, significantly facilitated 10-g-evoked responses of spinal neurones in neuropathic, but not sham-operated, rats. Spinal administration of UCM707 did not alter spinal levels of ECs but did significantly inhibit mechanically-evoked responses of neurones in neuropathic, but not sham-operated, rats. Pharmacological studies indicated that the selective inhibitory effects of spinal UCM707 in neuropathic rats were mediated by activation of spinal CB(2) receptors, as well as a contribution from transient receptor potential vanilloid 1 (TRPV1) channels. This work demonstrates that changes in the EC receptor system in the spinal cord of neuropathic rats influence the processing of sensory inputs, in particular low-weight inputs that drive allodynia, and indicates novel effects of drugs acting via multiple elements of this receptor system.
Databáze:	OpenAIRE
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