Sphingosine induces phospholipase D and mitogen activated protein kinase in vascular smooth muscle cells
| Autor: | Anwar S. Abd-Elfattah, Mohiuddin M. Taher, Milton M. Sholley |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Diacylglycerol Kinase
Time Factors Vascular smooth muscle Clinical Biochemistry Phosphatidic Acids Glycerophospholipids Biochemistry Muscle Smooth Vascular chemistry.chemical_compound Piperidines Sphingosine Phospholipase D Genetics Animals Molecular Biology Aorta Cells Cultured Protein Kinase C Protein kinase C Quinazolinones Diacylglycerol kinase Ethanol PLD2 Cell Biology Phosphatidic acid Lipid signaling Rats Enzyme Activation chemistry Type C Phospholipases Calcium-Calmodulin-Dependent Protein Kinases Quinazolines Tetradecanoylphorbol Acetate cGMP-dependent protein kinase Signal Transduction |
| Zdroj: | IUBMB Life. 46:993-1005 |
| ISSN: | 1521-6543 |
| DOI: | 10.1080/15216549800204552 |
| Popis: | The enzymes phospholipase D and diacylglycerol kinase generate phosphatidic acid which is considered to be a mitogen. Here we report that sphingosine produced a significant amount of phosphatidic acid in vascular smooth muscle cells from the rat aorta. The diacylglycerol kinase inhibitor R59 949 partially depressed sphingosine induced phosphatidic acid formation, suggesting that activation of phospholipase C and diacylglycerol kinase can not account for the bulk of phosphatidic acid produced and that additional pathways such as phospholipase D may contribute to this. Further, we have shown that phosphatidylethanol was produced by sphingosine when vascular smooth muscle cells were stimulated in the presence of ethanol. Finally, as previously shown for other cell types, sphingosine stimulated mitogen-activated protein kinase in vascular smooth muscle cells. |
| Databáze: | OpenAIRE |
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