C-reactive protein induces expression of matrix metalloproteinase-9: A possible link between inflammation and plaque rupture
| Autor: | Emanuela Falcinelli, Giovanni Cimmino, Gianluca Petrillo, Plinio Cirillo, Paolo Golino, Francesco Loffredo, Massimo Ragni, Massimo Chiariello, Paolo Gresele |
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| Přispěvatelé: | Cimmino, Giovanni, Ragni, M, Cirillo, P, Petrillo, G, Loffredo, F, Chiariello, M, Greselep, Falcinelli, E, Golino, Paolo, Cimmino, G, Cirillo, Plinio, Gresele, P, Golino, P. |
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Acute coronary syndrome Myocytes Smooth Muscle Inflammation Matrix metalloproteinase Gene Expression Regulation Enzymologic Muscle Smooth Vascular Pathogenesis Coronary circulation In vivo Internal medicine medicine.artery medicine Humans Acute Coronary Syndrome Cells Cultured Aged Acute coronary syndromes CRP MMP-9 Aorta biology business.industry C-reactive protein Middle Aged medicine.disease Plaque Atherosclerotic Up-Regulation C-Reactive Protein medicine.anatomical_structure Endocrinology Matrix Metalloproteinase 9 Immunology biology.protein Female medicine.symptom Cardiology and Cardiovascular Medicine business |
| Zdroj: | International Journal of Cardiology. 168:981-986 |
| ISSN: | 0167-5273 |
| DOI: | 10.1016/j.ijcard.2012.10.040 |
| Popis: | Background Matrix metalloproteases (MMPs) have been implicated in the pathogenesis of acute coronary syndromes (ACS). However, little is known about the mechanisms responsible for MMP expression in ACS. C-reactive protein (CRP) not only is an independent risk factor for cardiovascular events, but also may exert direct pro-atherosclerotic effects. Therefore, we aimed at determining whether CRP might induce MMP-9 in two different experimental conditions: 1) smooth muscle cells (SMCs) in vitro, and 2) patients with ACS. Methods and results Effects of increasing concentrations of CRP on MMP-9 expression were evaluated in vitro in human SMCs. TIMP-1 protein expression, the selective inhibitor of MMP-9, was also evaluated. CRP dose-dependently induced MMP-9 expression in SMCs by promoting MMP-mRNA transcription, as well as MMP-9 secretion. In contrast, no differences were found for TIMP-1 protein expression. In vivo, MMP-9 and CRP levels were measured in blood samples obtained from the aorta (Ao) and the coronary sinus (Cs) of patients with normal coronary arteries (controls, n=21), stable angina (n=24), and ACS (n=30). Both MMP-9 and CRP plasma levels were significantly increased across the coronary circulation only in patients with ACS. Interestingly, a significant correlation between MMP-9 and CRP plasma levels was found. Conclusions CRP induced MMP-9 expression and activity in human SMCs in culture; patients presenting with ACS have increased transcoronary plasma levels of MMP-9 and CRP with a significant correlation between these two markers. This may explain the heightened risk of coronary events in subjects with elevated levels of CRP. |
| Databáze: | OpenAIRE |
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