Apoptosis of rat hepatic stellate cells induced by diallyl trisulfide and proteomics profiling in vitro
| Autor: | Liu Qinghua, Jing Ye, Pengxin Qiu, Xiaoming Zhou, Lulu Wang, Jinling Liu, Lipeng Xu, Yajie Zhang |
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| Rok vydání: | 2017 |
| Předmět: |
Proteomics
0301 basic medicine Cell Survival Physiology Apoptosis Sulfides Biology Flow cytometry 03 medical and health sciences chemistry.chemical_compound Annexin Physiology (medical) Hepatic Stellate Cells medicine Animals Propidium iodide Garlic Cell Proliferation Pharmacology medicine.diagnostic_test Cell growth Cell Cycle General Medicine Molecular biology Rats Allyl Compounds 030104 developmental biology Diallyl trisulfide Gene Expression Regulation chemistry Hepatic stellate cell Bromodeoxyuridine |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 95:463-473 |
| ISSN: | 1205-7541 0008-4212 |
| Popis: | Diallyl trisulfide (DATS), a major garlic derivative, inhibits cell proliferation and triggers apoptosis in a variety of cancer cell lines. However, the effects of DATS on hepatic stellate cells (HSCs) remain unknown. The aim of this study was to analyze the effects of DATS on cell proliferation and apoptosis, as well as the protein expression profile in rat HSCs. Rat HSCs were treated with or without 12 and 24 μg/mL DATS for various time intervals. Cell proliferation and apoptosis were determined using tetrazolium dye (MTT) colorimetric assay, bromodeoxyuridine (5-bromo-2′-deoxyuridine; BrdU) assay, Hoechst 33342 staining, electroscopy, and flow cytometry. Protein expression patterns in HSCs were systematically studied using 2-dimensional electrophoresis and mass spectrometry. DATS inhibited cell proliferation and induced apoptosis of HSCs in a time-dependent manner. We observed clear morphological changes in apoptotic HSCs and dramatically increased annexin V-positive – propidium iodide negative apoptosis compared with the untreated control group. Twenty-one significant differentially expressed proteins, including 9 downregulated proteins and 12 upregulated proteins, were identified after DATS administration, and most of them were involved in apoptosis. Our results suggest that DATS is an inducer of apoptosis in HSCs, and several key proteins may be involved in the molecular mechanism of apoptosis induced by DATS. |
| Databáze: | OpenAIRE |
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