Purification, characterization and antibacterial spectrum of a compound produced by Bacillus cereus MTCC 10072
| Autor: | Jyoti Singh, Pallavi Thakur, Namita Singh, Narender Kumar, Chetna Bhandari, Anil Duhan, Rajneesh Jaryal |
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| Rok vydání: | 2019 |
| Předmět: |
Staphylococcus aureus
Chemical compound Bacillus cereus Bacillus Human pathogen Microbial Sensitivity Tests medicine.disease_cause Biochemistry Microbiology Mycobacterium 03 medical and health sciences Minimum inhibitory concentration chemistry.chemical_compound Bacterial Proteins Escherichia coli Genetics medicine Humans Pyrroles Food science Molecular Biology 030304 developmental biology 0303 health sciences biology 030306 microbiology Chemistry Streptococcus General Medicine biology.organism_classification Anti-Bacterial Agents Biological Control Agents Antibacterial activity Bacteria Bacillus subtilis |
| Zdroj: | Archives of Microbiology. 201:1195-1205 |
| ISSN: | 1432-072X 0302-8933 |
| Popis: | Awareness of the consumer has increased the demand of safe and chemical-free foods, and consequently it has increased the demand of antibacterial bioactive compounds. In the present study, antibacterial compound produced by a local bacterial isolate NSD MTCC 10072, showing antagonistic activity against six human pathogens, was isolated, partially purified and characterized. Maximum production of antibacterial compound was observed between 51 and 60 h after seeding. The antibacterial activity of the compound was found to be thermostable up to 80 °C for 60 min and its efficacy was very good between pH 4 and 12. Minimum inhibitory concentration (25.84 µg/µl) of the antibacterial compound was observed against Streptococcus aureus NICM 2901. GC–MS analysis of the bacterium secreted chemical compound (C11H18N2O2) was used to identify the antimicrobial compound as Pyrrolo(1,2-a) pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl). In Silico studies showed that the antimicrobial compound is non-toxic, non-irritating and followed Lipinski-type properties which suggested that the compound could be used as potential drug against different human pathogens. |
| Databáze: | OpenAIRE |
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