Galanin peptide levels in hippocampus and cortex of galanin-overexpressing transgenic mice evaluated for cognitive performance
| Autor: | Gary L. Wenk, Jefferson W. Kinney, Andrew Holmes, Craige C. Wrenn, Lisa K. Marriott, Jacqueline N. Crawley |
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| Rok vydání: | 2002 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Gene Expression Neuropeptide Morris water navigation task Hippocampus Galanin Mice Transgenic Hippocampal formation Food Preferences Mice Cellular and Molecular Neuroscience Cognition Endocrinology Memory Internal medicine Conditioning Psychological medicine Animals Fear conditioning Maze Learning Social Behavior Cerebral Cortex Endocrine and Autonomic Systems digestive oral and skin physiology Fear General Medicine Mice Inbred C57BL medicine.anatomical_structure nervous system Neurology Cerebral cortex Locus coeruleus Female Psychology Neuroscience hormones hormone substitutes and hormone antagonists |
| Zdroj: | Neuropeptides. 36:413-426 |
| ISSN: | 0143-4179 |
| Popis: | Galanin-overexpressing transgenic mice (GAL-tg) generated on a dopamine beta-hydroxylase promoter were previously shown to express high levels of galanin mRNA in the locus coeruleus, and to perform poorly on challenging cognitive tasks. The present study employed radioimmunoassay to quantitate the level of galanin peptide overexpression in two brain regions relevant to learning and memory, the hippocampus and cerebral cortex. Approximately 4-fold higher levels of galanin were detected in the hippocampus of GAL-tg as compared to WT. Approximately 10-fold higher levels of galanin were detected in the frontal cortex of GAL-tg as compared to WT. A second cohort of GAL-tg and WT again showed high levels of galanin overexpression in GAL-tg as compared to WT in both brain regions. Correlation analyses were conducted between galanin peptide concentrations and behavioral scores on four learning and memory tasks: the Morris water maze, social transmission of food preference, standard delay fear conditioning, and trace fear conditioning. While some significant correlations were detected, neither hippocampal nor cortical galanin levels in the two cohorts of GAL-tg consistently correlated with performance across these diverse cognitive tasks. Several interpretations of these findings are discussed, including the possibility that a threshold level of galanin overexpression is sufficient to impair performance on learning and memory tasks in mice. |
| Databáze: | OpenAIRE |
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