SPEECHLESS integrates brassinosteroid and stomata signalling pathways
| Autor: | Gustavo Eduardo Gudesblat, Walter van Dongen, Eugenia Russinova, Sacco C. de Vries, Joanna Schneider-Pizoń, Claudia Jonak, Isabelle Vanhoutte, Camilla Betti, Miroslava Zhiponova, Sjef Boeren, Juliane Mayerhofer |
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| Rok vydání: | 2011 |
| Předmět: |
Arabidopsis
Biochemie arabidopsis-thaliana Biology Brasinossinosteroids Biochemistry GSK3 Ciencias Biológicas chemistry.chemical_compound receptor kinases transcription factors Brassinosteroids Basic Helix-Loop-Helix Transcription Factors Brassinosteroid Arabidopsis thaliana Phosphorylation Protein kinase A Transcription factor Stomata Ciencias de las Plantas Botánica SPEECHLESS gsk3-like kinases EPS-1 Kinase Arabidopsis Proteins secretory peptide asymmetric cell-division differentiation phosphorylation sites Cell Biology biology.organism_classification Cell biology chemistry Plant Stomata identification biosynthesis Signal transduction Mitogen-Activated Protein Kinases CIENCIAS NATURALES Y EXACTAS Signal Transduction |
| Zdroj: | Nature Cell Biology, 14(5), 548-554 Nature Cell Biology 14 (2012) 5 |
| ISSN: | 1476-4679 1465-7392 |
| Popis: | Stomatal formation is regulated by multiple developmental and environmental signals, but how these signals are integrated to control this process is not fully understood. In Arabidopsis thaliana, the basic helix-loop-helix transcription factor SPEECHLESS (SPCH) regulates the entry, amplifying and spacing divisions that occur during stomatal lineage development. SPCH activity is negatively regulated by mitogen-activated protein kinase (MAPK)-mediated phosphorylation. Here, we show that in addition to MAPKs, SPCH activity is also modulated by brassinosteroid (BR) signalling. The GSK3/SHAGGY-like kinase BIN2 (BR INSENSITIVE2) phosphorylates residues overlapping those targeted by the MAPKs, as well as four residues in the amino-terminal region of the protein outside the MAPK target domain. These phosphorylation events antagonize SPCH activity and limit epidermal cell proliferation. Conversely, inhibition of BIN2 activity in vivo stabilizes SPCH and triggers excessive stomatal and non-stomatal cell formation. We demonstrate that through phosphorylation inputs from both MAPKs and BIN2, SPCH serves as an integration node for stomata and BR signalling pathways to control stomatal development in Arabidopsis. Fil: Gudesblat, Gustavo Eduardo. University of Ghent; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. Cesar Milstein". Fundación Pablo Cassara. Instituto de Ciencia y Tecnología ; Argentina Fil: Schneider Pizon, Joanna. University of Ghent; Bélgica Fil: Betti, Camilla. University of Ghent; Bélgica Fil: Mayerhofer, Juliane. Austrian Academy of Sciences; Austria Fil: Vanhoutte, Isabelle. University of Ghent; Bélgica Fil: Dongen, Walter van. Wageningen University; Países Bajos Fil: Boeren, Sjef. Wageningen University; Países Bajos Fil: Zhiponova, Miroslava. University of Ghent; Bélgica Fil: de Vries, Sacco. Wageningen University; Países Bajos Fil: Jonak, Claudia. Austrian Academy of Sciences; Austria Fil: Russinova, Eugenia. University of Ghent; Bélgica. University of Sofia; Bulgaria |
| Databáze: | OpenAIRE |
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