Primary and Secondary Infections by Human Parvovirus B19 following Bone Marrow Transplantation: Characterization by PCR and B-Cell Molecular Immunology
| Autor: | Kari Asikainen, Klaus Hedman, Petri Ruutu, Maria Söderlund, Tapani Ruutu, Rauli Franssila |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Adult
Male Microbiology (medical) Pathology medicine.medical_specialty viruses Secondary infection Biology Antibodies Viral Polymerase Chain Reaction Parvoviridae Infections 03 medical and health sciences 0302 clinical medicine hemic and lymphatic diseases Parvovirus B19 Human medicine Humans Avidity 030212 general & internal medicine Immunodeficiency Bone Marrow Transplantation Preparative Regimen B-Lymphocytes 0303 health sciences Leukopenia General Immunology and Microbiology 030306 microbiology Parvovirus virus diseases General Medicine Middle Aged medicine.disease biology.organism_classification 3. Good health Transplantation surgical procedures operative Infectious Diseases medicine.anatomical_structure Immunoglobulin M Immunoglobulin G Immunology Female Bone marrow medicine.symptom |
| Zdroj: | Scandinavian Journal of Infectious Diseases. 29:129-135 |
| ISSN: | 1651-1980 0036-5548 |
| DOI: | 10.3109/00365549709035872 |
| Popis: | Due to the preparative regimen necessary, bone marrow transplantation (BMT) consistently results in severe immunodeficiency, often associated with anaemia, leukopenia and thrombocytopenia. Parvovirus B19 replicates in red blood cell precursors in the bone marrow and causes erythema infectiosum ('fifth disease'), anaemia, arthritis and foetal death. We assessed the significance of B19 infections as a cause of post-BMT complications. Over 900 serial serum samples from 201 allogeneic bone marrow recipients were studied by polymerase chain reaction (PCR) and by modern serodiagnostic methods. During the first 6 months after transplantation all BMT recipients remained B19 PCR-negative. Antibody screening for B19 infections was performed up to 36 months post-transplantation. Three cases of acute B19 infection were diagnosed during the second year post-BMT. To characterize the adoptively transferred immune system we measured subclasses and avidity of anti-VP1 IgG and epitope-type specificity (ETS) of anti-VP2 IgG, which allowed functional differentiation of primary and secondary B-cell responses long after BMT. The profile of the immune response was that of a primary infection in 1 and of reinfection in 2 of the 3 acute cases. Both types were clinically mild. Infection by human parvovirus B19 is not a frequent cause of post-BMT cytopenias. The findings with the new B19 antibody markers support the concept that the donated marrow determines the type of antiviral B-cell responses. |
| Databáze: | OpenAIRE |
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