CRMP4 regulates dendritic growth and maturation via the interaction with actin cytoskeleton in cultured hippocampal neurons
| Autor: | Jifeng Zhang, Sitang Gong, Zhisheng Ji, Sumei Li, Hongsheng Lin, Guoqing Guo, Minghui Tan, Fengming Wu, Caihui Cha, Keen Chen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Dendritic spine Neurite Green Fluorescent Proteins Nerve Tissue Proteins macromolecular substances Biology Hippocampal formation Transfection Hippocampus Rats Sprague-Dawley 03 medical and health sciences Actin remodeling of neurons 0302 clinical medicine Animals Humans RNA Small Interfering Cells Cultured Actin Neurons General Neuroscience Intracellular Signaling Peptides and Proteins Excitatory Postsynaptic Potentials Membrane Proteins Dendrites Actin cytoskeleton Rats Dendritic filopodia Cell biology Actin Cytoskeleton HEK293 Cells 030104 developmental biology Animals Newborn Mutation Collapsin response mediator protein family Disks Large Homolog 4 Protein 030217 neurology & neurosurgery |
| Zdroj: | Brain Research Bulletin. 124:286-294 |
| ISSN: | 0361-9230 |
| DOI: | 10.1016/j.brainresbull.2016.06.008 |
| Popis: | CRMP family proteins (CRMPs) are critical for neurite outgrowth and maturation in the developing nervous system. However, the distinct roles of CRMP isoforms remain to be elucidated, especially in dendritic development. Here, we show that CRMP4 is sufficient and necessary for dendritic growth and maturation in cultured hippocampal neurons. Overexpression of CRMP4 promotes and genetic knockdown of CRMP4 inhibits the amount of dendritic tips, total dendritic length, spine density, and the frequency but not amplitude of miniature excitatory synaptic current. By GST-pulldown assay, we reveal that CRMP4 interacts with actin cytoskeleton by its C-terminal region, but not by N-terminal. Overexpression of actin-interacting region of CRMP4 promoted dendritic growth and maturation as CRMP4 wildtype. Taken together, these results suggest that CRMP4 is involved in dendritic development via the interaction with actin cytoskeleton in hippocampal neurons. |
| Databáze: | OpenAIRE |
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