An ATP-fueled nucleic acid signal amplification strategy for highly sensitive microRNA detection
| Autor: | Wen-Bin Liang, Chengyi Xiong, Ying Zhuo, Yaqin Chai, Zhi-Bin Wen, Ruo Yuan, Yingning Zheng |
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| Rok vydání: | 2018 |
| Předmět: |
Aptamer
010402 general chemistry 01 natural sciences Catalysis chemistry.chemical_compound Adenosine Triphosphate microRNA Fluorescence Resonance Energy Transfer Materials Chemistry Nucleotide chemistry.chemical_classification 010405 organic chemistry Chemistry Metals and Alloys General Chemistry Nucleic acid amplification technique Aptamers Nucleotide 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials MicroRNAs Förster resonance energy transfer Ceramics and Composites Nucleic acid Biophysics Nucleic Acid Amplification Techniques Signal amplification Adenosine triphosphate |
| Zdroj: | Chemical Communications. 54:10897-10900 |
| ISSN: | 1364-548X 1359-7345 |
| Popis: | Herein, an adenosine triphosphate (ATP)-fueled nucleic acid signal amplification strategy based on toehold-mediated strand displacement (TMSD) and fluorescence resonance energy transfer (FRET) was proposed for highly sensitive detection of microRNA-21. More importantly, the target microRNA-21 could be regenerated with ATP as the fuel rather than a nucleotide segment in conventional approaches, which made the proposed strategy simple and efficient due to the high affinity and strength of the aptamer-target interaction. |
| Databáze: | OpenAIRE |
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