Effect of Immunization With an Inactivated gp120-Depleted HIV-1 Immunogen on β-Chemokine and Cytokine Production in Subjects With HIV-1 Infection
| Autor: | Anne E. Daigle, Steven P. Richieri, Richard J. Trauger, Wieslawa K. Giermakowska, Georgia Theofan, Dennis J. Carlo, Ronald B. Moss, John Turner, Francois Ferre, Chris Duffy, Fred C. Jensen, Mark R. Wallace |
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| Rok vydání: | 1997 |
| Předmět: |
Cellular immunity
Chemokine Time Factors Immunogen medicine.medical_treatment Immunology HIV Infections Lymphocyte proliferation HIV Envelope Protein gp120 Biology Lymphocyte Activation Peripheral blood mononuclear cell Interferon-gamma Antigen Virology HIV Seropositivity medicine Humans Immunology and Allergy Chemokine CCL5 Cells Cultured CD4 Lymphocyte Count Cytokine Immunization HIV-1 biology.protein Cytokines Chemokines |
| Zdroj: | Journal of Acquired Immune Deficiency Syndromes & Human Retrovirology. 14:343-350 |
| ISSN: | 1077-9450 |
| DOI: | 10.1097/00042560-199704010-00006 |
| Popis: | OBJECTIVE To measure beta-chemokine and cytokine production in HIV-1-infected subjects undergoing treatment with HIV-1 immunogen (REMUNE). DESIGN Open label treatment study. METHODS beta-Chemokine and cytokine production in peripheral blood mononuclear cell (PBMC) culture. RESULTS Interferon-gamma production (p = 0.04) and lymphocyte proliferation (p = 0.001) to HIV-1 antigen-stimulated PBMCs increased after immunization with the HIV-1 immunogen. A correlation was demonstrated after immunization between HIV-1 antigen-stimulated lymphocyte proliferation and interferon-gamma levels (r = 0.53, p = 0.04). No significant change after immunization was seen for interleukin-4 production. A significant increase in mean levels of HIV-1 antigen-stimulated RANTES (i.e., regulated upon, activation normal T-cell expressed and secreted), was evident 1 month after immunization (p = 0.002) and remained elevated 3 months after immunization. RANTES production was decreased in CD8-depleted PBMC cultures. Mean serum HIV-1 RNA copy numbers and CD4 cell counts remained stable after immunization (p > 0.5). A correlation was demonstrated between HIV-1 antigen-stimulated interferon-gamma and RANTES production (r = 0.54, p = 0.002). CONCLUSIONS This report describes an augmentation of beta-chemokines and TH1-type cytokines from PBMCs after immunization with the HIV-1 immunogen. |
| Databáze: | OpenAIRE |
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