Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Biosensor
| Autor: | Christina Cordes, Carsten Harms, Walter Lang, Jorge Prada |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Resistive touchscreen cell heat-lysis Materials science Thermoplastic Cyclo-Olefin Copolymer Biocompatibility Microfluidics Nanotechnology lcsh:A biosensors Design for manufacturability chemistry fluorescence detection Copolymer RNA hybridization lcsh:General Works Biochip Biosensor |
| Zdroj: | Proceedings, Vol 2, Iss 13, p 810 (2018) |
| ISSN: | 2504-3900 |
| Popis: | This contribution outlines the design and manufacturing of a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval is carried on after bacteria heat-lysis by an on-chip micro-heater. Two additional carbon resistive temperature sensors printed on the biochip sealing film monitor the heating process. RNA is hybridized with capture probes on the reaction chamber surface and identification is achieved by detection of fluorescence tags. The application of the mentioned techniques and materials facilitates the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the potential of fully thermoplastic devices as biosensors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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