The peroxisomal transporter gene ANT1 is regulated by a deviant oleate response element (ORE): characterization of the signal for fatty acid induction
| Autor: | Andreas Hartig, Luigi Palmieri, Hanspeter Rottensteiner, Ralf Erdmann, Helmut Ruis, Barbara Hamilton, Aner Gurvitz |
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| Rok vydání: | 2002 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Genes Fungal Response element Saccharomyces cerevisiae Biology Biochemistry chemistry.chemical_compound Adenosine Triphosphate Genes Reporter Adenine nucleotide Peroxisomes Cycloheximide Promoter Regions Genetic Molecular Biology Protein Synthesis Inhibitors chemistry.chemical_classification Reporter gene Base Sequence Fatty Acids Fatty acid Cell Biology Peroxisome Lauric acid Oleic acid Lac Operon chemistry Nucleotide Transport Proteins Free fatty acid receptor Carrier Proteins Oleic Acid Plasmids Signal Transduction Research Article |
| Zdroj: | Biochemical Journal. 365:109-117 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj20011495 |
| Popis: | Saccharomyces cerevisiae ANT1/YPR128c encodes the peroxisomal adenine nucleotide transporter that provides ATP for intra-peroxisomal activation of medium-chain fatty acids. A lacZ reporter construct comprising the ANT1 promoter was shown to be comparatively more highly expressed in a wild-type strain grown on oleic acid, a long-chain fatty acid, than in pip2Delta(oaf1)Delta mutant cells that are defective in fatty acid induction. The ANT1 promoter was demonstrated to contain a deviant oleate response element (ORE) that could bind the Pip2p-Oaf1p transcription factor and confer activation on a basal CYC1-lacZ reporter gene. Expression of Ant1p as well as other enzymes whose genes are known to be regulated by a canonical ORE was found to be increased in cells grown on lauric acid, a medium-chain fatty acid. We concluded that the signal for induction does not differentiate between long- and medium-chain fatty acids. This signal was independent of beta-oxidation or the biogenesis of the peroxisomal compartment where this process occurs, since a pox1Delta strain blocked in the first and rate-limiting step of beta-oxidation as well as various pex mutant cells devoid of intact peroxisomes produced sufficient amounts of Pip2p-Oaf1p for binding OREs in vitro and for expressing an ORE-driven reporter gene. The signal's durability was shown to be related to the concentration of fatty acids in the medium, since a pex6Delta strain expressed an ORE-driven reporter gene at high levels for a longer period than did isogenic wild-type cells. Generation of the signal was also independent of protein synthesis, as demonstrated by cycloheximide treatment. |
| Databáze: | OpenAIRE |
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