Effects of long non-coding RNA uc.48+ on pain transmission in trigeminal neuralgia
| Autor: | Wei Xiong, Mengxia Tan, Shangdong Liang, Lingkun He, Lijuan Liu, Guodong Li, Yulin Shen, Cancan Yin, Shu Guan, Zhoujie Tong, Huixiang Ge, Yun Gao |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Diabetes Mellitus Experimental Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Diabetic Neuropathies Trigeminal neuralgia Internal medicine medicine Animals Phosphorylation RNA Small Interfering Gene knockdown Chemistry General Neuroscience RNA Purinergic signalling Trigeminal Neuralgia medicine.disease Rats Disease Models Animal 030104 developmental biology Endocrinology Trigeminal Ganglion Hyperalgesia Neuropathic pain Neuralgia RNA Long Noncoding Receptors Purinergic P2X7 Signal transduction 030217 neurology & neurosurgery Receptors Purinergic P2X3 |
| Zdroj: | Brain research bulletin. 147 |
| ISSN: | 1873-2747 |
| Popis: | Trigeminal neuralgia (TN) is the most common neuropathic pain in the facial area, for which the effective therapy is unavailable. Long non-coding RNA (lncRNA) such as lncRNA uc.48+ is involved in diabetic neuropathic pain and may affect purinergic signaling in ganglia of diabetic rats. In this research, chronic constriction injury of the infraorbital nerve (CCI-ION) was applied to establish a rat model of TN. Five days after local injection of siRNA targeting the lncRNA uc.48+ in trigeminal ganglia (TGs), the upregulated uc.48+ expression and the reduced mechanical withdrawal threshold (MWT) in the TN rats were significantly reversed. The expression of P2X7 receptor in TGs was increased in the TN group compared with the sham group, but uc.48+ siRNA treatment mitigated this effect. The phosphorylation of ERK1/2 in TGs of TN rats was significantly enhanced compared with the sham group, while uc.48+ siRNA treatment reversed this change. In addition, injection of the lncRNA uc.48+ overexpression plasmid in TGs of control rats significantly reduced the MWT but elevated the expression of P2X7 in TGs; the phosphorylation of ERK1/2 in TGs in these uc.48+-overexpressed rats was significantly higher, similar to the observations in rats of TN model. The interaction between uc.48+ and the P2X7 receptor was detected by RNA binding protein immunoprecipitation (RIP), indicating that P2X7 receptor could specifically bind to uc.48 + . In summary, knockdown of lncRNA uc.48+ by siRNA could inhibit transduction of TN signals, whereas uc.48+ overexpression promoted TN signal transduction. LncRNA uc.48+ may interact with P2X7 receptor to upregulate expression of P2X7 receptor and furthermore enhance the phosphorylation of ERK1/2 in TGs, thereby participating in pain transmission in TN. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect