Challenging tumour immunological techniques that help to track cancer stem cells in malignant melanomas and other solid tumours
| Autor: | Vanda Plótár, Beatrix Kotlan, Andras Szollar, Tímea Balatoni, Emil Farkas, Miklós Kásler, Istvan Vamosi-Nagy, Szabolcs Horvath, Klara Eles, Ákos Sávolt, Mihály Újhelyi, J. Tóth, Orsolya Csuka, Gabriella Liszkay, Laszlo S. Toth |
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| Rok vydání: | 2018 |
| Předmět: |
cancer stem cells
education.field_of_study Original Paper Phage display biology business.industry tumour immunology Population malignant melanomas Cancer medicine.disease technical developments Oncology Antigen Cancer stem cell Cancer cell biology.protein Cancer research Medicine Immunohistochemistry Radiology Nuclear Medicine and imaging Antibody business education |
| Zdroj: | Contemporary Oncology |
| ISSN: | 1428-2526 |
| Popis: | Aim of the study The arsenal of questions and answers about the minor cancer initiating cancer stem cell (CSC) population put responsible for cancer invasiveness and metastases, has left with an unsolved puzzle. Specific aims of a complex project were partly focused on revealing new biomarkers of cancer. We designed and set up novel techniques to facilitate the detection of cancerous cells. Materials and methods As a novel approach, we investigated B cells infiltrating breast carcinomas and melanomas (TIL-B) in terms of their tumour antigen binding potential. By developing the TIL-B phage display technology we provide here a new technology for the specific detection of highly tumour-associated antigens. Single chain Fv (scFv) antibody fragment phage ELISA, immunofluorescence (IF) FACS analysis, chamber slide technique with IF confocal laser microscopy and immunohistochemistry (IHC) in paraffin-embedded tissue sections were set up and standardized. Results We showed strong tumour-associated disialylated glycosphingolipid expression levels on various cancer cells using scFv antibody fragments, generated previously by uniquely invasive breast carcinoma TIL-B phage display library technology. Conclusions We report herein a novel strategy to obtain antibody fragments of human origin that recognise tumour-associated ganglioside antigens. Our investigations have the power to detect privileged molecules in cancer progression, invasiveness, and metastases. The technical achievements of this study are being harnessed for early diagnostics and effective cancer therapeutics. |
| Databáze: | OpenAIRE |
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