Cloning of nod gene regions from mesquite rhizobia and bradyrhizobia and nucleotide sequence of the nodD gene from mesquite rhizobia
| Autor: | K. F. Golly, Ross A. Virginia, P. M. Thomas, J. W. Zyskind |
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| Rok vydání: | 1995 |
| Předmět: |
DNA
Bacterial Rhizobiaceae Prosopis glandulosa Molecular Sequence Data Restriction Mapping medicine.disease_cause Applied Microbiology and Biotechnology Bradyrhizobium Polymerase Chain Reaction Rhizobium leguminosarum Rhizobia Symbiosis Bacterial Proteins Nitrogen Fixation Botany medicine Amino Acid Sequence Cloning Molecular DNA Primers Plants Medicinal Ecology biology Base Sequence Sequence Homology Amino Acid food and beverages Fabaceae biochemical phenomena metabolism and nutrition biology.organism_classification DNA-Binding Proteins Genes Bacterial Trans-Activators Rhizobium bacteria Food Science Biotechnology Bradyrhizobium japonicum Research Article |
| Zdroj: | Applied and environmental microbiology. 61(9) |
| ISSN: | 0099-2240 |
| Popis: | Nitrogen-fixing symbiosis between bacteria and the tree legume mesquite (Prosopis glandulosa) is important for the maintenance of many desert ecosystems. Genes essential for nodulation and for extending the host range to mesquite were isolated from cosmid libraries of Rhizobium (mesquite) sp. strain HW17b and Bradyrhizobium (mesquite) sp. strain HW10h and were shown to be closely linked. All of the cosmid clones of rhizobia that extended the host range of Rhizobium (Parasponia) sp. strain NGR234CS to mesquite also supported nodulation of a Sym- mesquite strain. The cosmid clones of bradyrhizobia that extended the host range of Rhizobium (Parasponia) sp. strain NGR234CS to mesquite were only able to confer nodulation ability in the Sym- mesquite strain if they also contained a nodD-hybridizing region. Subclones containing just the nodD genes of either genus did not extend the host range of Rhizobium (Parasponia) sp. to mesquite, indicating that the nodD gene is insufficient for mesquite nodulation. The nodD gene region is conserved among mesquite-nodulating rhizobia regardless of the soil depth from which they were collected, indicating descent from a common ancestor. In a tree of distance relationships, the NodD amino acid sequence from mesquite rhizobia clusters with homologs from symbionts that can infect both herbaceous and tree legumes, including Rhizobium tropici, Rhizobium leguminosarum bv; phaseoli, Rhizobium loti, and Bradyrhizobium japonicum. |
| Databáze: | OpenAIRE |
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