The Effects of Subchronic Methionine Overload Administered Alone or Simultaneously with L-cysteine or N-acetyl-L-cysteine on Body Weight, Homocysteine Levels and Biochemical Parameters in the Blood of Male Wistar Rats
| Autor: | Dragan Djuric, Marija Stojanović, Adi Hadzibegovic, Sanja Stankovic, Radmila Obrenovic, Biljana Jakovljevic, Zarko Micovic, Ljiljana Šćepanović, Tamara Nikolic, Marko Djuric, Aleksandra Stamenkovic, Ivana Vujosevic |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Hyperhomocysteinemia Sodium l-cysteine chemistry.chemical_element Homocysteine levels Pathogenesis 03 medical and health sciences chemistry.chemical_compound Basal (phylogenetics) 0302 clinical medicine Internal medicine N-acetyl-L-cysteine Mole Medicine rat L-cysteine hyperhomocysteinemia methionine 2. Zero hunger Methionine business.industry n-acetyl-l-cysteine General Medicine medicine.disease 3. Good health 030104 developmental biology Endocrinology Biochemistry chemistry 030211 gastroenterology & hepatology business Cysteine |
| Zdroj: | Serbian Journal of Experimental and Clinical Research (2016) 17(3):215-223 Serbian Journal of Experimental and Clinical Research, Vol 17, Iss 3, Pp 215-224 (2016) |
| ISSN: | 2335-075X 1820-8665 |
| DOI: | 10.1515/sjecr-2016-0017 |
| Popis: | Hyperhomocysteinemia (HHC), both basal and after methionine load, may occur due to genetic disorders or deficiencies of nutrients that affect the remethylation or trans-sulphuration pathways during methionine metabolism. HHC is involved in the pathogenesis of many illnesses as a result of its prooxidative effect and its impairment of antioxidative protection. The aim was to examine the effects of subchronic methionine overload on the body weight and standard biochemical parameters in rat serum and to examine whether simultaneous subchronic intraperotoneal administration of methionine alone or together with L-cysteine or N-acetyl-cysteine resulted in a change in the body weight and biochemical parameters in the rat serum. The research was conducted during a three-week period (male Wistar albino rats, n=36, body weight of approximately 160 g, age of 15-20 days), and the animals were divided into a control group and three experimental groups of 8-10 animals each: a) control group (0.9% sodium chloride 0.1-0.2 ml/day); b) methionine (0.8 mmol/kg/bw/day) (MET group); c) methionine (0.8 mmol/kg/bw/day) + L-cysteine (7 mg/kg/bw/day) (L-cys+MET group); and d) methionine (0.8 mmol/kg/bw/day) + N-acetyl-L-cysteine (50 mg/kg/bw/day) (NAC+MET group). In addition to the body weight monitoring, the levels of total homocysteine and the standard biochemical parameters in blood samples (plasma or serum) were determined. The results indicated that monitoring the homocysteine levels and standard biochemical parameters in blood could be used for analysis and could provide an excellent guideline for distinguishing between toxic and non-toxic doses of methionine intake, which may be meaningful for clinical applications. |
| Databáze: | OpenAIRE |
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