Structural and functional characterization of toxic peptides purified from the venom of the Colombian scorpion Tityus macrochirus
Autor: | Fernando Zamudio Zuñiga, Edmundo González Santillán, Edgar A. Reyes-Montaño, Edson Norberto Carcamo-Noriega, Lourival D. Possani, Clara Andrea Rincón-Cortés, Nohora Angélica Vega Castro, Timoteo Olamendi-Portugal |
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Rok vydání: | 2019 |
Předmět: |
Centruroides
Tityus macrochirus Venenos de Escorpión Scorpion Scorpion Venoms Peptide Venom Toxicology medicine.disease_cause Secuencia de Aminoácidos Amino acid sequence Gryllidae Scorpions Canales de Sodio Sequence Analysis Protein biology.animal Toxicity Tests medicine Animals Phylogeny chemistry.chemical_classification Molecular mass biology Phylogenetic tree Sodium-channels Chemistry Toxin Péptidos biology.organism_classification Scorpion toxin Amino acid Biochemistry Female Peptides |
Zdroj: | Repositorio Institucional UDCA Universidad de Ciencias Aplicadas y Ambientales U.D.C.A instacron:Universidad de Ciencias Aplicadas y Ambientales U.D.C.A |
ISSN: | 1879-3150 |
Popis: | The soluble venom of the scorpion Tityus macrochirus was separated by chromatographic procedures and three homogeneous peptides were obtained and their primary structures were determined. They were called: Tma1-Tma3, from the abbreviated name of the scorpion. Tma1 is a peptide containing 65 amino acids with four disulfide linkages and a molecular weight of 7386.2 Da. It is a mammalian toxin, shown to affect human sodium-channels sub-types hNav1.6 and hNav1.4. Tma2 and Tma3 are peptides containing 69 amino acids linked by four disulfide bonds, molecular weights 7819.7 and 7830.0 Da, respectively. They do not affect human sodium-channels but are lethal to insects (crickets). A phylogenic analysis of the three peptides and those of other toxic peptides isolated from the genus Tityus and Centruroides were grouped together and analyzed, permitting to obtain a topology with two main clades, one includes most sodium-channel anti-insect scorpion toxins and others includes mostly sodium-channel scorpion toxins anti-mammalian. Tma1 segregates among a group of well-studied β-class toxins of other Tityus species such as T. discrepans, T. obscurus and T. pachyurus. Tma2 and Tma3 are associated with anti-insect toxins, particularly with one of T. obscurus. This phylogenetic analysis confirms and enforces our experimental results obtained with these three new sodium-channel scorpion toxins. |
Databáze: | OpenAIRE |
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