Diacylglycerol Acetyltransferase Gene Isolated from Euonymus europaeus L. Altered Lipid Metabolism in Transgenic Plant towards the Production of Acetylated Triacylglycerols
| Autor: | Pavol Hauptvogel, Ján Kraic, Jana Moravčíková, Daniel Mihálik, Simona Grešíková, Z. Šubr, Andrea Lančaričová, Michaela Mrkvová, Richard Hančinský, Šarlota Kaňuková, Lukáš Predajňa, Miroslav Glasa, Michaela Havrlentová |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Transgene 01 natural sciences General Biochemistry Genetics and Molecular Biology DGAT Celastraceae 03 medical and health sciences Euonymus Euonymus europaeus lcsh:Science Gene Ecology Evolution Behavior and Systematics biology DAcT Nucleic acid sequence Paleontology food and beverages biology.organism_classification Transformation (genetics) 030104 developmental biology Biochemistry Space and Planetary Science Acetyltransferase acetylTAG lcsh:Q 010606 plant biology & botany |
| Zdroj: | Life, Vol 10, Iss 205, p 205 (2020) Life Volume 10 Issue 9 |
| ISSN: | 2075-1729 |
| Popis: | Euonymus species from the Celastraceae family are considered as a source of unusual genes modifying the oil content and fatty acid composition of vegetable oils. Due to the possession of genes encoding enzyme diacylglycerol acetyltransferase (DAcT), Euonymus plants can synthesize and accumulate acetylated triacyglycerols. The gene from Euonymus europaeus (EeDAcT) encoding the DAcT was identified, isolated, characterized, and modified for cloning and genetic transformation of plants. This gene has a unique nucleotide sequence and amino acid composition, different from orthologous genes from other Euonymus species. Nucleotide sequence of original EeDAcT gene was modified, cloned into transformation vector, and introduced into tobacco plants. Overexpression of EeDAcT gene was confirmed, and transgenic host plants produced and accumulated acetylated triacylglycerols (TAGs) in immature seeds. Individual transgenic plants showed difference in amounts of synthesized acetylTAGs and also in fatty acid composition of acetylTAGs. |
| Databáze: | OpenAIRE |
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