Biochemical activities of 6-carboxy β-chitin derived from squid pens
| Autor: | Wan-wen Chen, Hong-Wu Lai, Sheng Hu, Jianwei Mao, Jun Huang, Jing-Yan Gong, Lehe Mei, Peng Liu |
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| Rok vydání: | 2013 |
| Předmět: |
Bromides
Antioxidant Polymers and Plastics Sodium Hypochlorite medicine.drug_class medicine.medical_treatment Radical Carboxylic Acids Chitin macromolecular substances Bile acid binding Iron Chelating Agents Ferrous Bile Acids and Salts Cyclic N-Oxides chemistry.chemical_compound Picrates Animal Shells Materials Chemistry medicine Animals Organic chemistry Chelation Carboxylate Bile acid Hydroxyl Radical Biphenyl Compounds fungi Organic Chemistry Decapodiformes Free Radical Scavengers Sodium Compounds carbohydrates (lipids) chemistry Titration Oxidation-Reduction Nuclear chemistry |
| Zdroj: | Carbohydrate Polymers. 91:191-197 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2012.08.027 |
| Popis: | TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical)-mediated 6-carboxy β-chitin derivatives (T-chitin) with different carboxylate content were successfully synthesized by controlling the addition level of NaClO as the primary oxidant. The structural and biochemical properties of the derivatives were investigated. The carboxylate contents of the derivatives calculated by electrical conductivity titration were 1.33, 1.68, 1.80, and 2.08 mmol/g, respectively. The yield of T-chitin with carboxylate content of 2.08 mmol/g reached 74.55%. T-chitin exhibited stronger bile acid binding capacities than that of β-chitin. The scavenging ability of T-chitin against hydroxyl radicals improved with increasing concentration, and EC(50) values were below 1.2 mg/mL. All T-chitin exhibited a strong ferrous ion chelating effect. At 8 mg/mL, the chelating effects of T-chitin with carboxylate content of 0.81 mmol/g reached 80.15%. These results showed that T-chitin had good bile acid binding capacity and antioxidant activities and it may be a potential antioxidant in vitro. |
| Databáze: | OpenAIRE |
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