Circulating exosomal microRNAs in acquired aplastic anemia and myelodysplastic syndromes
| Autor: | Sachiko Kajigaya, Maria del Pilar Fernandez Ibanez, Danielle M. Townsley, Fernanda Gutierrez-Rodrigues, Reema Panjwani, Olga Rios, Christopher K. E. Bleck, Neal S. Young, Erin Stempinski, Lauren G. Banaszak, Diego Quinones Raffo, Valentina Giudice |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Anemia Exosomes Exosome Article 03 medical and health sciences 0302 clinical medicine microRNA medicine Humans Circulating MicroRNA Aplastic anemia business.industry Myelodysplastic syndromes Gene Expression Profiling Case-control study Anemia Aplastic Biomarkers Case-Control Studies Computational Biology MicroRNAs Myelodysplastic Syndromes Prognosis Reproducibility of Results Aplastic Hematology Bone Marrow Failure medicine.disease 030104 developmental biology 030220 oncology & carcinogenesis Cancer cell Cancer research business |
| Zdroj: | Haematologica |
| Popis: | Exosomal microRNAs modulate cancer cell metabolism and the immune response. Specific exosomal microRNAs have been reported to be reliable biomarkers of several solid and hematologic malignancies. We examined the possible diagnostic and prognostic values of exosomal microRNAs in two human bone marrow failure diseases: aplastic anemia and myelodysplastic syndromes. After screening 372 microRNAs in a discovery set (n=42) of plasma exosome samples, we constructed a customized PCR plate, including 42 microRNAs, for validation in a larger cohort (n=99). We identified 25 differentially expressed exosomal microRNAs uniquely or frequently present in aplastic anemia and/or myelodysplastic syndromes. These microRNAs could be related to intracellular functions, such as metabolism, cell survival, and proliferation. Clinical parameters and progression-free survival were correlated to microRNA expression levels in aplastic anemia and myelodysplastic syndrome patients before and after six months of immunosuppressive therapy. One microRNA, mir-126-5p, was negatively correlated with a response to therapy in aplastic anemia: patients with higher relative expression of miR-126-5p at diagnosis had the shortest progression-free survival compared to those with lower or normal levels. Our findings suggest utility of exosomal microRNAs in the differential diagnosis of bone marrow failure syndromes. (Registered at clinicaltrials.gov identifiers: 00260689, 00604201, 00378534, 01623167, 00001620, 00001397, 00217594). |
| Databáze: | OpenAIRE |
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