Genistein enhances expression of extracellular regulated kinases (ERK) 1/2, and learning and memory of mouse
| Autor: | Paramanik Vijay, Kurrey Khuleshwari |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
MAPK/ERK pathway
medicine.medical_specialty Immunoblotting Estrogen receptor Genistein Neurosciences. Biological psychiatry. Neuropsychiatry Biology Open field Learning and memory chemistry.chemical_compound Internal medicine Extracellular medicine ERK1/2 Kinase General Neuroscience Neurogenesis Brain Novel Object Recognition Endocrinology chemistry Neuron differentiation RC321-571 Research Paper |
| Zdroj: | IBRO Neuroscience Reports IBRO Neuroscience Reports, Vol 10, Iss, Pp 90-95 (2021) |
| ISSN: | 2667-2421 |
| Popis: | Genistein (GEN) is a well known phytoestrogen. It acts through estrogen receptor (ER) and performs plethora of functions in the brain. ERK1/2 is an activated kinase which involves in neuron differentiation, adult neurogenesis and several brain functions including learning and memory. However, GEN dependent expression of ERK1/2 and its effect in learning and memory of mice are unknown. In this study, Swiss albino male mice of 25weeks weighing 30 g were used for the experiments. Mice were placed in two groups- control (C) and genistein treated (GEN). Treated group received GEN dissolved in sesame oil (1 mg/kg/day) whereas the control group received sesame oil only. To study the effects of GEN on learning and memory, open-field (OF) test and novel object recognition (NOR) test were performed. Moreover, immunoblotting (IB) was performed to check the expression of ERK1/2 in the mouse brain of both groups. In the OF test, no significant change was observed in motor activity and anxiety in GEN treated mice as compared to control. Moreover, NOR test suggested that entry towards the dissimilar object was higher in case of GEN treated mice as compared to control. These findings suggest higher learning and memory of GEN treated mice than of control. IB showed that the expression of ERK1/2 was significantly high in GEN treated mouse brain as compared to control. Such study may be helpful to understand GEN mediated learning and memory involving ERK1/2. Highlights • The present paper reports that GENISTEIN enhances learning and memory of mouse at behavioral level. • GENISTEIN enhances ERK1/2 expression which is manifested at behavioral level. • The present paper links between the GENISTEIN and ERK1/2 from molecular level to behavioral level in the context of learning and memory. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|