Comparison of disc diffusion methods for the detection of extended-spectrum Beta lactamase-producing enterobacteriaceae
| Autor: | B. V. S. Krishna, M R Chandrasekhar, Namratha W Nandihal, Asha B Patil, Ravi S Giriyapur |
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| Rok vydání: | 2011 |
| Předmět: |
0301 basic medicine
Screening test medicine.medical_treatment NCCLS screening 030106 microbiology 030231 tropical medicine lcsh:Medicine Ceftazidime Microbiology 03 medical and health sciences 0302 clinical medicine Enterobacteriaceae β lactams medicine DDST Diffusion methods biology β lactamases lcsh:R biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses World wide ESBLs Beta-lactamase Original Article PCT medicine.drug |
| Zdroj: | Journal of Laboratory Physicians Journal of Laboratory Physicians, Vol 3, Iss 01, Pp 033-036 (2011) |
| ISSN: | 0974-7826 |
| Popis: | Background: Resistance to broad-spectrum β lactams, mediated by extended-spectrum β lactamases (ESBLs), is an increasing problem world wide. This resistance poses problems for in vitro testing and reporting. Increased prevalence of ESBLs among Enterobacteriaceae creates a great need for laboratory testing methods that will accurately identify their presence.Materials and Methods: During the study, the Enterobacteriaceae isolated were tested for the presence of ESBL by the National Committee for Clinical Laboratory Standards (NCCLS) screening test, Jarlier double disc synergy (approximation) test (DDST) and NCCLS phenotypic confirmatory test (PCT), and compared their efficiency in detection.Results: A total of 313 Enterobacteriaceae were isolated and tested for the presence of ESBL. NCCLS PCT identified 200 (63.89%) as ESBL producers and DDST identified 176 (56.23%), with a P-value of Conclusions: Close monitoring of the susceptibility pattern of isolates and careful spacing with specific discs can identify many ESBL producers. Ceftazidime has a better sensitivity and specificity as a screening agent. A combination of different tests can be useful for accurate identification. |
| Databáze: | OpenAIRE |
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