Quail-chick grafting experiments corroborate that Tbr1-positive eminential prethalamic neurons migrate along three streams into hypothalamus, subpallium and septocommissural areas
| Autor: | Carmen Maria Trujillo, Luis Puelles, Antonia Alonso |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Telencephalon
Histology Lateral hypothalamus Prethalamic eminence Hypothalamus Chick Embryo In situ hybridization Biology Quail Cell Movement biology.animal medicine Animals Diencephalon Neurons Cerebrum General Neuroscience Cell Differentiation Tbr1 Cell biology Preoptic area medicine.anatomical_structure Diagonal band Quail-chick chimeras Extended amygdala biology.protein Original Article Commissural septum TBR1 Anatomy Chickens Heterochrony |
| Zdroj: | Brain Structure & Function |
| ISSN: | 1863-2661 1863-2653 |
| DOI: | 10.1007/s00429-020-02206-3 |
| Popis: | The prethalamic eminence (PThE), a diencephalic caudal neighbor of the telencephalon and alar hypothalamus, is frequently described in mammals and birds as a transient embryonic structure, undetectable in the adult brain. Based on descriptive developmental analysis ofTbr1gene brain expression in chick embryos, we previously reported that three migratory cellular streams exit the PThE rostralward, targeting multiple sites in the hypothalamus, subpallium and septocommissural area, where eminential cells form distinct nuclei or disperse populations. These conclusions needed experimental corroboration. In this work, we used the homotopic quail-chick chimeric grafting procedure at stages HH10/HH11 to demonstrate by fate-mapping the three predicted tangential migration streams. Some chimeric brains were processed forTbr1in situ hybridization, for correlation with our previous approach. Evidence supporting all three postulated migration streams is presented. The results suggested a slight heterochrony among the juxtapeduncular (first), the peripeduncular (next), and the eminentio-septal (last) streams, each of which followed differential routes. A possible effect of such heterochrony on the differential selection of medial to lateral habenular hodologic targets by the migrated neurons is discussed. |
| Databáze: | OpenAIRE |
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