Methylation of SFRPs and APC Genes in Ovarian Cancer Infected with High Risk Human Papillomavirus
| Autor: | Mohamed M. Sayed-Ahmed, Mohamed M. Hafez, Salem S. Al-Rejaie, Zeinab K. Hassan, Abdulmalik Alsheikh, Othman A. Al-Shabanah, Waleed Abozeed |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Cancer Research Epidemiology Adenomatous polyposis coli Adenomatous Polyposis Coli Protein Bisulfite sequencing Young Adult Risk Factors Proto-Oncogene Proteins medicine Humans Promoter Regions Genetic Papillomaviridae Gene Aged Glycoproteins Neoplasm Staging Aged 80 and over Ovarian Neoplasms Regulation of gene expression biology Papillomavirus Infections Intracellular Signaling Peptides and Proteins Public Health Environmental and Occupational Health Membrane Proteins Cancer Methylation DNA Methylation Middle Aged Prognosis medicine.disease Molecular biology Gene Expression Regulation Neoplastic Oncology DNA methylation biology.protein Intercellular Signaling Peptides and Proteins Female Ovarian cancer Follow-Up Studies |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 15:2719-2725 |
| ISSN: | 1513-7368 |
| DOI: | 10.7314/apjcp.2014.15.6.2719 |
| Popis: | Background: Secreted frizzled-related protein (SFRP) genes, new tumor suppressor genes, are negative regulators of the Wnt pathway whose alteration is associated with various tumors. In ovarian cancer, SFRPs genes promoter methylation can lead to gene inactivation. This study investigated mechanisms of SFRP and adenomatous polyposis coli (APC) genes silencing in ovarian cancer infected with high risk human papillomavirus. Materials and Methods: DNA was extracted from 200 formalin-fixed paraffin-embedded ovarian cancer and their normal adjacent tissues (NAT) and DNA methylation was detected by methylation specific PCR (MSP). High risk human papillomavirus (HPV) was detected by nested PCR with consensus primers to amplify a broad spectrum of HPV genotypes. Results: The percentages of SFRP and APC genes with methylation were significantly higher in ovarian cancer tissues infected with high risk HPV compared to NAT. The methylated studied genes were associated with suppression in their gene expression. Conclusion: This finding highlights the possible role of the high risk HPV virus in ovarian carcinogenesis or in facilitating cancer progression by suppression of SFRP and APC genes via DNA methylation. |
| Databáze: | OpenAIRE |
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