Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale
| Autor: | Gisele Bacanelli, Stenio Perdigão Fragoso, Maristeli B. Oliveira, Lenita Ramires dos Santos, Carlos Alberto do Nascimento Ramos, Roberto Augusto de Almeida Torres Júnior, Nalvo F. Almeida, Paulo R. C. Arauco, Cleber O. Soares, Graziela S. Araújo, Flábio R. Araújo, Leucio Câmara Alves, Cláudio R. Madruga, Raul H. Kessler, Valeska Shelda Pessoa de Melo |
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| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Anaplasmosis lcsh:Arctic medicine. Tropical medicine indirect enzyme-linked immunosorbent assays lcsh:RC955-962 lcsh:QR1-502 Cattle Diseases Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Biology Sensitivity and Specificity lcsh:Microbiology law.invention Microbiology Kappa index Anaplasma marginale law major surface proteins parasitic diseases Animals chemistry.chemical_classification Antigens Bacterial biology.organism_classification Antibodies Bacterial Virology Recombinant Proteins Enzyme Rickettsia chemistry Recombinant DNA biology.protein Cattle Antibody Brazil Bacterial Outer Membrane Proteins |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 100, Iss 7, Pp 765-769 (2005) Memórias do Instituto Oswaldo Cruz, Volume: 100, Issue: 7, Pages: 765-769, Published: NOV 2005 |
| ISSN: | 0074-0276 |
| DOI: | 10.1590/s0074-02762005000700016 |
| Popis: | Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. The high sensitivities (99% for both tests) and specificities (100% for both tests) were confirmed with sera from cattle positive or negative for A. marginale antibodies, respectively, by immunofluorescent antibody test. By the analysis of 583 sera from cattle of three regions of the state of Pernambuco, Brazil, the agreement between both tests was high, with a kappa index of 0.89. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle. |
| Databáze: | OpenAIRE |
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