Structure of the Toll/interleukin 1 receptor (TIR) domain of the immunosuppressive Brucella effector BtpA/Btp1/TcpB

Autor: Christine Felix, Marie-Pierre Candusso, Laurent Terradot, David O'Callaghan, Thomas Koelblen, Burcu Kaplan-Türköz, Annette C. Vergunst
Přispěvatelé: ERGonomie de l'Activité des Professionnels de l'Education (ERGAPE), Apprentissage, Didactique, Evaluation, Formation (ADEF), Aix Marseille Université (AMU)-Aix Marseille Université (AMU), School of Computer Science and Statistics [Dublin], Trinity College Dublin, Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Models
Molecular
Dimer
[SDV]Life Sciences [q-bio]
Biochemistry
Virulence factor
chemistry.chemical_compound
MESH: Protein Structure
Tertiary
MESH: Structure-Activity Relationship
Structural Biology
Receptor
MESH: Bacterial Proteins
0303 health sciences
Effector
MESH: Protein Multimerization
030302 biochemistry & molecular biology
3. Good health
Cell biology
BtpA
MESH: Models
Molecular
Virulence Factors
Biophysics
Brucella
Interleukin-1 receptor
Biology
MESH: Receptors
Interleukin-1
digestive system
03 medical and health sciences
Structure-Activity Relationship
Bacterial Proteins
Microtubule
TLR
parasitic diseases
Genetics
Brucella melitensis
Humans
MESH: Paracoccus denitrificans
Molecular Biology
030304 developmental biology
X-ray crystallography
Paracoccus denitrificans
MESH: Virulence Factors
MESH: Humans
TIR domain
Receptors
Interleukin-1
Cell Biology
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Protein Structure
Tertiary
Crystallography
MESH: Brucella melitensis
chemistry
Protein Multimerization
Structural mimicry
Zdroj: FEBS Letters
FEBS Letters, 2013, 587 (21), pp.3412-6. ⟨10.1016/j.febslet.2013.09.007⟩
FEBS Letters, Wiley, 2013, 587 (21), pp.3412-6. ⟨10.1016/j.febslet.2013.09.007⟩
ISSN: 0014-5793
1873-3468
DOI: 10.1016/j.febslet.2013.09.007⟩
Popis: International audience; BtpA/Btp1/TcpB is a virulence factor produced by Brucella species that possesses a Toll interleukin-1 receptor (TIR) domain. Once delivered into the host cell, BtpA interacts with MyD88 to interfere with TLR signalling and modulates microtubule dynamics. Here the crystal structure of the BtpA TIR domain at 3.15 Å is presented. The structure shows a dimeric arrangement of a canonical TIR domain, similar to the Paracoccus denitrificans Tir protein but secured by a unique long N-terminal α-tail that packs against the TIR:TIR dimer. Structure-based mutations and multi-angle light scattering experiments characterized the BtpA dimer conformation in solution. The structure of BtpA will help with studies to understand the mechanisms involved in its interactions with MyD88 and with microtubules.
Databáze: OpenAIRE
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