Genotoxic effects in C57Bl/6J mice chronically exposed to arsenate in drinking water and modulation of the effects by low-selenium diet
| Autor: | Dobrosława Lewińska, Maciej Stępnik, Jolanta Gromadzinska, Elżbieta Dziubałtowska, Wojciech Wasowicz, Jadwiga Palus, Joanna Arkusz, Małgorzata Stańczyk, Konrad Rydzyński, Małgorzata Trzcinka-Ochocka |
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| Rok vydání: | 2006 |
| Předmět: |
Purine
medicine.medical_specialty Health Toxicology and Mutagenesis chemistry.chemical_element Administration Oral Toxicology C57bl 6j Antioxidants chemistry.chemical_compound Mice Selenium Water Supply Internal medicine medicine Animals Drug Interactions chemistry.chemical_classification Mutagenicity Tests Arsenate Diet Comet assay Mice Inbred C57BL Oxidative Stress Endocrinology Enzyme Teratogens chemistry Biochemistry Micronucleus test DNA fragmentation Arsenates Female DNA Damage |
| Zdroj: | Journal of toxicology and environmental health. Part A. 69(20) |
| ISSN: | 1528-7394 |
| Popis: | In C57Bl/6J mice chronically exposed to arsenate in drinking water at 50, 200, or 500 microg As/L, genotoxic effects in bone-marrow cells using micronucleus test and in peripheral blood leukocytes using the comet assay were determined after 3, 6 or 12 mo. To assess the modulating role of selenium in development of the effects, the animals were fed a specially prepared low-selenium diet and were supplemented with sodium selenite (200 microg Se/L) in drinking water (supplemented groups) or were without Se supplementation (nonsupplemented groups). Measurements of glutathione peroxidase activity in erythrocytes and plasma as well as selenium concentration in plasma were performed after 3, 6, and 12 mo and showed a marked decrease in values in animals in non-Se supplemented compared to Se-supplemented groups. After 3 mo of arsenic exposure in the Se-supplemented animals the level of DNA fragmentation (without Endo III and Fpg enzymes) did not differ from the control; however, increased oxidative damage of purine and pyrimidine bases was observed. In groups not supplemented with Se, an increase of DNA fragmentation was observed; however, the levels of oxidative DNA damage in these groups did not differ from the control. None of the positive effects observed in the comet assay after 3 mo was related to arsenate concentration. The levels of DNA damage after 6 and 12 mo of exposure to arsenic as well as the frequency of micronuclei after 3, 6, and 12 mo did not differ significantly between exposed and control animals, irrespective of Se supplementation status. |
| Databáze: | OpenAIRE |
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