Characterization of mitochondrial ferritin in Drosophila
| Autor: | Sara Holmberg, Fanis Missirlis, T. Georgieva, Boris C. Dunkov, Tracey A. Rouault, John H. Law |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Male
Molecular Sequence Data Mitochondrion chemistry.chemical_compound Mice Biosynthesis Testis Animals Drosophila Proteins Humans Amino Acid Sequence Heme Gene Multidisciplinary Genome biology Sequence Homology Amino Acid fungi MITOCHONDRIAL FERRITIN Transfection Biological Sciences biology.organism_classification Mitochondria Ferritin Protein Subunits Drosophila melanogaster chemistry Biochemistry Gene Expression Regulation Ferritins biology.protein Female Sequence Alignment |
| Popis: | Mitochondrial function depends on iron-containing enzymes and proteins, whose maturation requires available iron for biosynthesis of iron–sulfur clusters and heme. Little is known about how mitochondrial iron homeostasis is maintained, although the recent discovery of a mitochondrial ferritin in mammals and plants has uncovered a potential key player in the process. Here, we show that Drosophila melanogaster expresses mitochondrial ferritin from an intron-containing gene. It has high similarity to the mouse and human mitochondrial ferritin sequences and, as in mammals, is expressed mainly in testis. This ferritin contains a putative mitochondrial targeting sequence and an epitope-tagged version localizes to mitochondria in transfected cells. Overexpression of mitochondrial ferritin fails to alter both total-body iron levels and iron that is bound to secretory ferritins. However, the viability of iron-deficient flies is compromised by overexpression of mitochondrial ferritin, suggesting that it may sequester iron at the expense of other important cellular functions. The conservation of mitochondrial ferritin in an insect species underscores the importance of this iron-storage molecule. |
| Databáze: | OpenAIRE |
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