A modified form of low-density lipoprotein with increased electronegative charge is present in rheumatoid arthritis synovial fluid
| Autor: | Paul G. Winyard, D R Blake, Zhi Zhang, Karl Gaffney, Letian Dai, Christopher Morris, Hugh W. Jones |
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| Rok vydání: | 1997 |
| Předmět: |
Male
medicine.medical_specialty Inflammation Osteoarthritis Biochemistry Pathogenesis Arthritis Rheumatoid chemistry.chemical_compound Physiology (medical) Internal medicine Synovial Fluid medicine Electrochemistry Synovial fluid Humans Aged chemistry.chemical_classification Reactive oxygen species Middle Aged medicine.disease Lipoproteins LDL Endocrinology chemistry Low-density lipoprotein Rheumatoid arthritis Immunology lipids (amino acids peptides and proteins) Female medicine.symptom Reactive Oxygen Species Immunoelectrophoresis Two-Dimensional Oxidation-Reduction Lipoprotein |
| Zdroj: | Free radical biologymedicine. 22(4) |
| ISSN: | 0891-5849 |
| Popis: | Reactive oxygen species (ROS) are pro-inflammatory factors in the pathogenesis of rheumatoid arthritis. During inflammation, the amount of low-density lipoprotein (LDL) in the inflamed joint is increased. LDL is known to be susceptible to oxidation by ROS. Oxidized LDL may serve as a mediator for joint damage, further exacerbating the inflammatory process. LDL isolated from synovial fluid and plasma from individual patients (paired samples) with rheumatoid arthritis or osteoarthritis was characterized by crossed immunoelectrophoresis. On analysis by this technique, synovial fluid LDL from most patients with rheumatoid arthritis contained two peaks: one corresponding to normal plasma native LDL, and the other having an increased electrophoretic mobility associated with oxidized LDL. Paired plasma LDL samples contained native LDL alone, as did paired synovial fluid and plasma LDL from patients with osteoarthritis. Thus, in addition to native LDL, a second form of LDL was shown to be present in rheumatoid synovial fluid, which had properties consistent with those of oxidized LDL. |
| Databáze: | OpenAIRE |
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