Protective effects of Salvia plebeia compound homoplantaginin on hepatocyte injury
Autor: | Jianjun Gao, Xue Xia, Yan-Ying Xie, Xiang-Juan Liu, Xiao-Fan Zhang, Xian-Jun Qu, Lan Xiang, Yuan-Shu Wang, Li-Li Liu, Shu-Xiang Cui, Lu-Lu Qiu, Mei-Juan Song, Yan-Na Cheng |
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Rok vydání: | 2008 |
Předmět: |
Necrosis
Cell Survival Pharmacology Toxicology Protective Agents Thiobarbituric Acid Reactive Substances Antioxidants Cell Line Superoxide dismutase chemistry.chemical_compound Mice Glucosides Liver Function Tests medicine TBARS Animals Humans Salvia Liver injury chemistry.chemical_classification Flavonoids biology L-Lactate Dehydrogenase Tumor Necrosis Factor-alpha Glutathione peroxidase General Medicine Glutathione Hydrogen Peroxide medicine.disease biology.organism_classification Enzymes Oxidative Stress medicine.anatomical_structure chemistry Biochemistry Liver Hepatocyte biology.protein Hepatocytes medicine.symptom Chemical and Drug Induced Liver Injury Salvia plebeia Food Science Interleukin-1 |
Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 47(7) |
ISSN: | 1873-6351 |
Popis: | Salvia plebeia R. Br is a traditional Chinese herb which has been considered as an inflammatory mediator used for treatment of many infectious diseases including hepatitis. Previously, the compound homoplantaginin was isolated in our group. Hence, we evaluated the protective effects of homoplantaginin on hepatocyte injury. Homoplantaginin displayed an antioxidant property in a cell-free system and showed IC(50) of reduction level of DPPH radical at 0.35 microg/ml. In human hepatocyte HL-7702 cells exposed to H(2)O(2), the addition of 0.1-100 microg/ml of homoplantaginin, which did not have a toxic effect on cell viability, significantly reduced lactate dehydrogenase (LDH) leakage, and increased glutathione (GSH), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in supernatant. In vivo assay, we employed the model of Bacillus Calmette-Guerin (BCG)/lipopolysaccharide (LPS)-induced hepatic injury mice to evaluate efficacy of homoplantaginin. Homoplantaginin (25-100mg/kg) significantly reduced the increase in serum alanine aminotranseferase (ALT) and aspartate aminotransferase (AST), decreased the levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1). The same treatment also reduced the content of thiobarbituric acid-reactive substances (TBARS), elevated the levels of GSH, GSH-Px and SOD in hepatic homogenate. The histopathological analysis showed that the grade of liver injury was ameliorated with reduction of inflammatory cells and necrosis of liver cells in homoplantaginin treatment mice. These results suggest that homoplantaginin has a protective and therapeutic effect on hepatocyte injury, which might be associated with its antioxidant properties. |
Databáze: | OpenAIRE |
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