Photo-Cross-Linked Hydrogels with Polysaccharide−Poly(amino acid) Structure: New Biomaterials for Pharmaceutical Applications
| Autor: | Fabio Salvatore Palumbo, Gaetano Giammona, Giovanna Pitarresi, Paola Pierro, Giuseppe Tripodo |
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| Přispěvatelé: | PITARRESI G, PIERRO P, PALUMBO FS, TRIPODO G, GIAMMONA G |
| Rok vydání: | 2006 |
| Předmět: |
Time Factors
Polymers and Plastics Ultraviolet Rays Chemistry Pharmaceutical Molecular Sequence Data Methacrylic anhydride Biocompatible Materials Bioengineering macromolecular substances Biomaterials chemistry.chemical_compound Polysaccharides Polymer chemistry Carbohydrate Conformation Materials Chemistry Amino Acids chemistry.chemical_classification Aqueous solution Chemistry technology industry and agriculture Chemical modification Hydrogels Amino acid Carbohydrate Sequence biomaterials drug delivery hyaluronic acid Drug delivery Self-healing hydrogels Liberation Drug carrier Nuclear chemistry |
| Zdroj: | Biomacromolecules. 7:1302-1310 |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/bm050697m |
| Popis: | The aim of this work has been the preparation and characterization of novel hydrogels with polysaccharide-poly(amino acid) structure having suitable physicochemical properties for pharmaceutical applications. In the first step, hyaluronic acid (HA) and alpha,beta-poly(N-2-hydroxyethyl)-DL-aspartamide (PHEA) have been derivatized with methacrylic anhydride (AMA), thus obtaining HA-AMA and PHM derivatives, respectively. In the second step, aqueous solutions of both these derivatives have been irradiated at 313 nm to obtain chemical hydrogels. The hydrogel obtained by irradiating for 15 min an aqueous solution containing 4% w/v of HA-AMA and 4% w/v of PHM resulted in the highest yield. Its swelling ability was dependent on the pH and nature of the external medium. Besides, this hydrogel undergoes a partial hydrolysis, especially in the presence of enzymes, such as esterase or hyaluronidase, but the entity of this degradation is lower than that observed for a hydrogel based on HA-AMA alone. The ability of this hydrogel to entrap drug molecules has been evaluated by using thrombin as a model drug. In vitro release studies and a platelet aggregation test demonstrated that the HA-AMA/PHM hydrogel is able to release thrombin in the active form, thus suggesting its suitability for the treatment of hemorrhages. |
| Databáze: | OpenAIRE |
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