Sialic acids in gastropods
| Autor: | Sabine Bürgmayr, Erika Staudacher, Heidi Grabher-Meier |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
N-glycolylneuraminic acid
Arion lusitanicus Biophysics Sialidase medicine.disease_cause Biochemistry Gas Chromatography-Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound Structural Biology N-Glycolylneuraminic acid Genetics medicine Animals Gastropod Molecular Biology Chromatography High Pressure Liquid Skin 030304 developmental biology 0303 health sciences Chromatography biology Chemistry 030302 biochemistry & molecular biology Lectin Cell Biology Clostridium perfringens biology.organism_classification Sialic acid Mollusca Sialic Acids biology.protein Gas chromatography N-acetylneuraminic acid N-Acetylneuraminic acid |
| Zdroj: | FEBS Letters. 508:95-98 |
| ISSN: | 0014-5793 |
| Popis: | The occurrence of N-acetylneuraminic acid and N-glycolylneuraminic acid residues in preparations of the slug Arion lusitanicus (Gastropoda) was determined by sodium dodecyl sulphate electrophoresis of the proteins followed by lectin blots stained with the sialic acid specific lectin from Maackia amurensis, by the sensitivity of this binding to sialidase from Clostridium perfringens, by specific fluorescent labelling of sialic acids with 1,2-diamino-4,5-methylenedioxybenzene, by the determination of the sensitivity to sialate-pyruvate-lyase, by co-migration with standards on high performance anion exchange chromatography with pulsed amperometric detection and by identification of the typical masses in the fragmentation patterns of the trimethylsilyl derivatives after gas chromatography. It is the first time sialic acids are identified in gastropods. |
| Databáze: | OpenAIRE |
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