Involvement of DnaK3, One of the Three DnaK Proteins of CyanobacteriumSynechococcussp. PCC7942, in Translational Process on the Surface of the Thylakoid Membrane
| Autor: | Yoko Katano, Kaori Nimura-Matsune, Hirofumi Yoshikawa |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Ribosomal Proteins
Molecular Sequence Data Mutant Thylakoids Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Bacterial Proteins Ribosomal protein Polysome Protein biosynthesis HSP70 Heat-Shock Proteins Amino Acid Sequence Molecular Biology 50S Synechococcus Base Sequence biology Organic Chemistry Membrane Proteins Photosystem II Protein Complex General Medicine biology.organism_classification Polyribosomes Protein Biosynthesis Thylakoid Chaperone (protein) Mutation biology.protein Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 70:1592-1598 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.50631 |
| Popis: | The Synechococcus sp. PCC7942 strain carrying a missense mutation in the peptide-binding domain of DnaK3, one of the essential dnaK gene products, revealed temperature-sensitive growth. We also isolated suppressor mutants of this strain. One of the suppressors was mapped in the ribosomal protein gene rpl24 (syc1876), which encodes the 50S ribosomal protein L24. Subcellular localization of three DnaK proteins was determined, and the results indicated that a quantity of DnaK3 was dislocated from membrane-bound polysomes when dnaK3 temperature-sensitive mutant was incubated at non-permissive temperatures. Furthermore, we examined the photosystem II reaction center protein D1 and detected a translational intermediate polypeptide in membrane-bound polysome fractions prepared from dnaK3 temperature-sensitive cells grown at high temperature. These characteristic features of DnaK3 localizations and detection of D1 protein intermediate were not observed in the suppressor mutant even at high temperatures. |
| Databáze: | OpenAIRE |
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