Retroposition in a family of carcinoma-associated antigen genes
| Autor: | Alban Linnenbach, T. Druck, M. Scollon, S. Robbins, Shuang Wu, B. A. Seng, J. J. Pyrc, Kay Huebner |
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| Rok vydání: | 1993 |
| Předmět: |
Molecular Sequence Data
Pair-rule gene Biology Exon shuffling Exon Gene mapping Complementary DNA Gene cluster Humans Gene family Antigens Tumor-Associated Carbohydrate Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Gene Genetics Base Sequence Sequence Homology Amino Acid Genome Human Chromosome Mapping Sequence Analysis DNA Cell Biology Biological Evolution Molecular biology Chromosomes Human Pair 1 Multigene Family DNA Transposable Elements Chromosomes Human Pair 4 Research Article |
| Zdroj: | Molecular and Cellular Biology. 13:1507-1515 |
| ISSN: | 1098-5549 0270-7306 |
| Popis: | The gene encoding the carcinoma-associated antigen defined by the monoclonal antibody GA733 is a member of a family of at least two type I membrane proteins. This study describes the mechanism of evolution of the GA733-1 and GA733-2 genes. A full-length cDNA clone for GA733-1 was obtained by screening a human placental library with a genomic DNA probe. Comparative analysis of the cDNA sequence with the previously determined genomic sequence confirmed that GA733-1 is an intronless gene. The GA733-2 gene encoding the monoclonal antibody-defined antigen was molecularly cloned with a cDNA probe and partially sequenced. Comparison of GA733-2 gene sequences with the previously established cDNA sequence revealed that this gene consists of nine exons. The putative promoter regions of the GA733-1 and GA733-2 genes are unrelated. These findings suggest that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate. Prior to retroposition, the GA733-2 gene had been affected by exon shuffling. Analysis of GA733-2 exons revealed that many delineate structural motifs. The GA733-1 retroposon was localized either to chromosome region 1p32-1p31 or to 1p13-1q12, and the GA733-2 founder gene was localized to chromosome 4q. |
| Databáze: | OpenAIRE |
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