Association of the -1031TC polymorphism and soluble TNF-α levels with Acute Coronary Syndrome

Autor: Lorena Michele Brennan-Bourdon, Emmanuel Valdés-Alvarado, Héctor Enrique Flores-Salinas, Ilian Janet García-González, Angélica Valdez-Haro, Jorge Ramón Padilla-Gutiérrez, Yeminia Valle, Elena Sandoval-Pinto, José Francisco Muñoz-Valle
Rok vydání: 2015
Předmět:
0301 basic medicine
Male
Acute coronary syndrome
medicine.medical_specialty
Genotype
medicine.medical_treatment
Immunology
030204 cardiovascular system & hematology
Biochemistry
Gastroenterology
Polymerase Chain Reaction
Polymorphism
Single Nucleotide
Proinflammatory cytokine
03 medical and health sciences
0302 clinical medicine
Gene Frequency
Risk Factors
Internal medicine
medicine
Immunology and Allergy
Humans
Genetic Predisposition to Disease
Allele
Acute Coronary Syndrome
Promoter Regions
Genetic
Molecular Biology
Allele frequency
Alleles
Genetic Association Studies
Aged
Dyslipidemias
business.industry
Tumor Necrosis Factor-alpha
Case-control study
Hematology
Middle Aged
medicine.disease
030104 developmental biology
Cytokine
Case-Control Studies
Female
business
Dyslipidemia
Polymorphism
Restriction Fragment Length
Zdroj: Cytokine. 78
ISSN: 1096-0023
Popis: Inflammation has gained a pivotal role in the pathophysiology of Acute Coronary Syndrome (ACS). TNF-α is a pro-inflammatory cytokine that could be a potential biomarker in ACS due to its multiple functions. The rs1799964 TNFA polymorphism (-1031TC) has been associated with a decrease in gene transcription and cytokine levels.To determine the association of rs1799964 TNFA polymorphism and TNF-α soluble levels in ACS.A total of 251 patients diagnosed with ACS and 164 individuals without cardiovascular diseases classified as the reference group (RG), were included. The rs1799964 polymorphism was genotyped by PCR-RFLP. Soluble protein levels were determined by ELISA. Statistical analyses were performed using chi square and U-Mann Whitney tests.The genotype and allele frequencies were different between ACS and RG (OR=0.317, p=0.01; OR=0.688, p=0.03 respectively). ACS patients had higher soluble TNF-α levels compared with the RG (31.08 vs 23.00pg/mL, p0.001); according genotype significant differences were observed (T/T: 24.06 vs T/C: 34.95pg/mL, p=0.0001) in patients. In the RG, T/T carriers showed discrete lower levels than C/C genotype (22.14 vs 27.83pg/mL, p=0.04).The -1031C allele of the TNFA polymorphism confers protection for the development of ACS. The T/C genotype carriers had higher TNF-α serum levels compared to the T/T genotype in ACS. In addition, the -1031TC TNFA polymorphism was associated with dyslipidemia in ACS in a Western Mexican population.
Databáze: OpenAIRE
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